Acceleration of Bone Regeneration in Critical-Size Defect Using BMP-9-Loaded nHA/ColI/MWCNTs Scaffolds Seeded with Bone Marrow Mesenchymal Stem Cells

Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for...

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Bibliographic Details
Published in:BioMed research international 2019-01, Vol.2019 (2019), p.1-10
Main Authors: Zhu, Tong, Gao, Xiaobo, Liu, Yao, Li, Xuewen, Zhang, Ran, Lu, Li
Format: Article
Language:English
Subjects:
Acceleration
Biocompatibility
Biomedical materials
Biomedical research
Bone growth
Bone marrow
Bone morphogenetic protein 9
Carbon
Cell adhesion & migration
Collagen
Collagen (type I)
Freeze drying
Hydroxyapatite
Mechanical properties
Mesenchymal stem cells
Mesenchyme
Multi wall carbon nanotubes
Osteoblastogenesis
Osteoblasts
Osteogenesis
Porosity
Proteins
Regeneration
Regeneration (physiology)
Scaffolding
Scaffolds
Stem cells
Surgical implants
Tissue engineering
Transplants & implants
Vaccines
Water absorption
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Summary:Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for bone tissue engineering by in vitro and in vivo experiments. The composite nHA/ColI/MWCNT scaffolds were fabricated at various concentrations of MWCNTs (0.5, 1, and 1.5% wt) by blending and freeze drying. The porosity, swelling rate, water absorption rate, mechanical properties, and biocompatibility of scaffolds were measured. After loading with BMP-9, bone marrow mesenchymal stem cells (BMMSCs) were seeded to evaluate their characteristics in vitro and in a critical sized defect in Sprague-Dawley rats in vivo. It was shown that the 1% MWCNT group was the most suitable for bone tissue engineering. Our results demonstrated that scaffolds loaded with BMP-9 promoted differentiation of BMMSCs into osteoblasts in vitro and induced more bone formation in vivo. To conclude, nHA/ColI/MWCNT scaffolds loaded with BMP-9 possess high biocompatibility and osteogenesis and are a good candidate for use in bone tissue engineering.
ISSN:2314-6133
2314-6141
DOI:10.1155/2019/7343957