Green Tea Polyphenols Modify the Gut Microbiome in db/db Mice as Co‐Abundance Groups Correlating with the Blood Glucose Lowering Effect

• Published in: Molecular nutrition & food research 2019-04, Vol.63 (8), p.e1801064-n/a
• Main Authors: Chen, Tingting, Liu, Anna B., Sun, Shili, Ajami, Nadim J., Ross, Matthew C., Wang, Hong, Zhang, Le, Reuhl, Kenneth, Kobayashi, Koichi, Onishi, Janet C., Zhao, Liping, Yang, Chung S.
• Format: Article
• Language: English
• Subjects: Animals; Beta cells; Biflavonoids - pharmacology; Black tea; Blood; Blood glucose; Blood Glucose - metabolism; Body Weight - drug effects; Catechin - analogs & derivatives; Catechin - pharmacology; Cecum; Colon; Community structure; db/db mice; diabetes; Diabetes mellitus; Dietary supplements; Female; Gastrointestinal Microbiome - drug effects; Gastrointestinal Microbiome - genetics; Gastrointestinal Microbiome - physiology; Gene sequencing; Glucose; Green tea; Hypoglycemic Agents - pharmacology; Ileum; Insulin; Insulin - blood; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - pathology; intestinal microbiota; Intestinal microflora; Levels; metabolism; Mice, Mutant Strains; Microbiomes; Microbiota; Organ Size - drug effects; Polyphenols; Polyphenols - pharmacology; RNA, Ribosomal, 16S; rRNA 16S; Tea; Tea - chemistry; tea polyphenols; Theaflavins; Trinucleotide repeats
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.201801064

Scope The effects of green tea polyphenols, Polyphenon E (PPE), and black tea polyphenols, theaflavins (TFs), on gut microbiota and development of diabetes in db/db mice are investigated and compared. Methods and results Supplementation of PPE (0.1%) in the diet of female db/db mice for 7 weeks decreases fasting blood glucose levels and mesenteric fat while increasing the serum level of insulin, possibly through protection against β‐cell damage. However, TFs are less or not effective. Microbiome analysis through 16S rRNA gene sequencing shows that PPE and TFs treatments significantly alter the bacterial community structure in the cecum and colon, but not in the ileum. The key bacterial phylotypes responding to the treatments are then clustered into 11 co‐abundance groups (CAGs). CAGs 6 and 7, significantly increased by PPE but not by TFs, are negatively associated with blood glucose levels. The operational taxonomic units in these CAGs are from two different phyla, Firmicutes and Bacteroidetes. CAG 10, decreased by PPE and TFs, is positively associated with blood glucose levels. Conclusion Gut microbiota respond to tea polyphenol treatments as CAGs instead of taxa. Some of the CAGs associated with the blood glucose lowering effect are enriched by PPE, but not TFs. Dietary supplementation of green tea Polyphenon E (PPE) to db/db mice for seven weeks decreases fasting blood glucose levels. It also alters gut microbiota in co‐abundance groups (CAGs). CAGs 6 and 7 (increased by PPE) are negatively correlated with blood glucose levels while CAG10 (decreased by PPE) is positively correlated with blood glucose levels. Black tea theaflavins (TFs) also decrease CAG10, but have little effect on CAGs 6 and 7 as well as on blood glucose levels.