In Vitro Activity of a Novel Glycopolymer against Biofilms of Burkholderia cepacia Complex Cystic Fibrosis Clinical Isolates

complex (Bcc) lung infections in cystic fibrosis (CF) patients are often associated with a steady decline in lung function and death. The formation of biofilms and inherent multidrug resistance are virulence factors associated with Bcc infection and contribute to increased risk of mortality in CF pa...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2019-06, Vol.63 (6)
Main Authors: Narayanaswamy, Vidya P, Duncan, Andrew P, LiPuma, John J, Wiesmann, William P, Baker, Shenda M, Townsend, Stacy M
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Anti-Bacterial Agents - pharmacology
Biofilms
Biofilms - drug effects
Burkholderia cepacia complex
Burkholderia cepacia complex - drug effects
Burkholderia Infections
Burkholderia Infections - drug therapy
Burkholderia Infections - microbiology
Cystic Fibrosis
Cystic Fibrosis - drug therapy
Cystic Fibrosis - microbiology
Glucosamine
Glucosamine - pharmacology
Humans
Mechanisms of Resistance
Microbial Sensitivity Tests - methods
Respiratory Tract Infections - drug therapy
Respiratory Tract Infections - microbiology
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Summary:complex (Bcc) lung infections in cystic fibrosis (CF) patients are often associated with a steady decline in lung function and death. The formation of biofilms and inherent multidrug resistance are virulence factors associated with Bcc infection and contribute to increased risk of mortality in CF patients. New therapeutic strategies targeting bacterial biofilms are anticipated to enhance antibiotic penetration and facilitate resolution of infection. Poly (acetyl, arginyl) glucosamine (PAAG) is a cationic glycopolymer therapeutic being developed to directly target biofilm integrity. In this study, 13 isolates from 7 species were examined, including , , , , , and These isolates were selected for their resistance to standard clinical antibiotics and their ability to form biofilms Biofilm biomass was quantitated using static tissue culture plate (TCP) biofilm methods and a minimum biofilm eradication concentration (MBEC) assay. Confocal laser scanning microscopy (CLSM) visualized biofilm removal by PAAG during treatment. Both TCP and MBEC methods demonstrated a significant dose-dependent relationship with regard to biofilm removal by 50 to 200 μg/ml PAAG following a 1-h treatment (  
ISSN:0066-4804
1098-6596
1098-6596
DOI:10.1128/AAC.00498-19