HDAC5 Expression in Urothelial Carcinoma Cell Lines Inhibits Long-Term Proliferation but Can Promote Epithelial-to-Mesenchymal Transition

• Published in: International journal of molecular sciences 2019-04, Vol.20 (9), p.2135
• Main Authors: Jaguva Vasudevan, Ananda Ayyappan, Hoffmann, Michèle J, Beck, Michael L C, Poschmann, Gereon, Petzsch, Patrick, Wiek, Constanze, Stühler, Kai, Köhrer, Karl, Schulz, Wolfgang A, Niegisch, Günter
• Format: Article
• Language: English
• Subjects: Bladder; Bladder cancer; Cancer; Carcinoma - genetics; Carcinoma - metabolism; Cases (containers); Cell adhesion & migration; Cell growth; Cell Line, Tumor; Cell morphology; Cell Proliferation; Cloning; Cluster analysis; Cytology; Enzymes; Epithelial-Mesenchymal Transition; Gene expression; Genomes; HEK293 Cells; Histone Deacetylases - genetics; Histone Deacetylases - metabolism; Humans; Invasiveness; Mesenchyme; Missense mutation; Morphology; Mutation; Phenotypes; Proteins; Proteomics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tumor cell lines; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urothelial carcinoma; Urothelium - metabolism; Urothelium - pathology
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20092135

Class I histone deacetylases (HDACs) generally promote cell proliferation and tumorigenesis, whereas class IIA HDACs like HDAC4 and HDAC5 may promote or impede cancer development in a tissue-dependent manner. In urothelial carcinoma (UC), HDAC5 is often downregulated. Accordingly, HDAC5 was weakly expressed in UC cell lines suggesting a possible tumor-suppressive function. We therefore characterized the effects of stable HDAC5 expression in four UC cell lines (RT112, VM-Cub-1, SW1710 and UM-UC-3) with different phenotypes reflecting the heterogeneity of UC, by assessing proliferation, clonogenicity and migration ability. Further, we detailed changes in the proteome and transcriptome by immunoblotting, mass spectrometry and RNA sequencing analysis. We observed that HDAC5 overexpression in general decreased cell proliferation, but in one cell line (VM-Cub-1) induced a dramatic change from an epitheloid to a mesenchymal phenotype, i.e., epithelial-mesenchymal transition (EMT). These phenotypical changes were confirmed by comprehensive proteomics and transcriptomics analyses. In contrast to HDAC5, overexpression of HDAC4 exerted only weak effects on cell proliferation and phenotypes. We conclude that overexpression of HDAC5 may generally decrease proliferation in UC, but, intriguingly, may induce EMT on its own in certain circumstances.