Protein kinase A inhibits tumor mutator APOBEC3B through phosphorylation

• Published in: Scientific reports 2019-06, Vol.9 (1), p.8307, Article 8307
• Main Authors: Matsumoto, Tadahiko, Shirakawa, Kotaro, Yokoyama, Masaru, Fukuda, Hirofumi, Sarca, Anamaria Daniela, Koyabu, Sukenao, Yamazaki, Hiroyuki, Kazuma, Yasuhiro, Matsui, Hiroyuki, Maruyama, Wataru, Nagata, Kayoko, Tanabe, Fumiko, Kobayashi, Masayuki, Shindo, Keisuke, Morishita, Ryo, Sato, Hironori, Takaori-Kondo, Akifumi
• Format: Article
• Language: English
• Subjects: 13/1; 42/70; 45/23; 45/77; 631/337/458/1733; 631/67/395; 82/29; 82/51; 82/83; 96/109; 96/33; Breast cancer; Catalytic Domain; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits - metabolism; Cytidine deaminase; Cytidine Deaminase - antagonists & inhibitors; Cytidine Deaminase - genetics; Cytoplasm - metabolism; Cytosine; Cytosine - chemistry; Deoxyribonucleic acid; DNA; DNA, Single-Stranded - genetics; Fluorescence Resonance Energy Transfer; HEK293 Cells; HeLa Cells; Humanities and Social Sciences; Humans; Infectivity; Kinases; Minor Histocompatibility Antigens - genetics; Molecular dynamics; Molecular Dynamics Simulation; multidisciplinary; Multiple myeloma; Mutation; Neoplasms - enzymology; Neoplasms - genetics; Phosphorylation; Protein kinase A; Retrotransposition; Science; Science (multidisciplinary); Threonine; Threonine - chemistry; Tumors; Uracil
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-44407-9

APOBEC3B cytidine deaminase (A3B) catalyzes cytosine into uracil in single-strand DNA and induces C-to-T mutations in genomic DNA of various types of tumors. Accumulation of APOBEC signature mutations is correlated with a worse prognosis for patients with breast cancer or multiple myeloma, suggesting that A3B activity might be a cause of the unfavorable DNA mutations and clonal evolution in these tumors. Phosphorylation of conserved threonine residues of other cytidine deaminases, activation induced deaminase (AID) and APOBEC3G, inhibits their activity. Here we show that protein kinase A (PKA) physically binds to A3B and phosphorylates Thr214. In vitro deaminase assays and foreign DNA editing assays in cells confirm that phosphomimetic A3B mutants, T214D and T214E, completely lose deaminase activity. Molecular dynamics simulation of A3B phosphorylation reveals that Thr214 phosphorylation disrupts binding between the phospho-A3B catalytic core and ssDNA. These mutants still inhibit retroviral infectivity at least partially, and also retain full anti-retrotransposition activity. These results imply that PKA-mediated phosphorylation inhibits A3B mutagenic activity without destructing its innate immune functions. Therefore, PKA activation could reduce further accumulation of mutations in A3B overexpressing tumors.