Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem Cells

Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during d...

Full description

Saved in:
Bibliographic Details
Published in:International journal of stem cells 2019-07, Vol.12 (2), p.240-250
Main Authors: Choi, Seon-A, An, Ju-Hyun, Lee, Seung Hwan, Lee, Geun-Hui, Yang, Hae-Jun, Jeong, Pil-Soo, Cha, Jae-Jin, Lee, Sanghoon, Park, Young-Ho, Song, Bong-Seok, Sim, Bo-Woong, Kim, Young-Hyun, Kim, Ji-Su, Jin, Yeung Bae, Huh, Jae-Won, Lee, Sang-Rae, Lee, Jong-Hee, Kim, Sun-Uk
Format: Article
Language:English
Subjects:
Original
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17- (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. We used 10 nM E2, 3 M P4, 10 nM DM, and 10 nM RA based on their functional developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34 CD45 cells with progenitor functions, even in the CD43 population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications.
ISSN:2005-3606
2005-5447
DOI:10.15283/ijsc18137