Retinoid-related orphan receptor alpha controls the early steps of Purkinje cell dendritic differentiation

Dendritic differentiation involves both regressive and growth events. The mechanisms controlling the regressive events are poorly understood. This study is aimed at determining the role of the nuclear receptor retinoid-related orphan receptor alpha (RORalpha) in Purkinje cell (PC) dendritic differen...

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Bibliographic Details
Published in:The Journal of neuroscience 2006-02, Vol.26 (5), p.1531-1538
Main Authors: Boukhtouche, Fatiha, Janmaat, Sonja, Vodjdani, Guilan, Gautheron, Vanessa, Mallet, Jacques, Dusart, Isabelle, Mariani, Jean
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cognitive Sciences
Dendrites - ultrastructure
Genetic Vectors
Kinetics
Lentivirus - genetics
Life Sciences
Mice
Mice, Neurologic Mutants
Neurons and Cognition
Nuclear Receptor Subfamily 1, Group F, Member 1
Organ Culture Techniques
Purkinje Cells - cytology
Purkinje Cells - metabolism
Receptor Protein-Tyrosine Kinases
Receptor Tyrosine Kinase-like Orphan Receptors
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Receptors, Cytoplasmic and Nuclear - deficiency
Receptors, Cytoplasmic and Nuclear - genetics
Receptors, Cytoplasmic and Nuclear - physiology
Trans-Activators - deficiency
Trans-Activators - genetics
Trans-Activators - physiology
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Summary:Dendritic differentiation involves both regressive and growth events. The mechanisms controlling the regressive events are poorly understood. This study is aimed at determining the role of the nuclear receptor retinoid-related orphan receptor alpha (RORalpha) in Purkinje cell (PC) dendritic differentiation in organotypic cultures. As observed in vivo, in these cultures, fusiform PCs with embryonic bipolar shape undergo regression before the outgrowth of the ultimate dendritic tree. We show that lentiviral-mediated hRORalpha1 overexpression in fusiform PCs leads to a cell-autonomous accelerated progression of dendritic differentiation. In addition, RORalpha is necessary for the PC regressive events: whereas staggerer RORalpha-deficient PCs remain in the embryonic fusiform stage, replacement of hRORalpha1 restores normal dendritogenesis. These results demonstrate that RORalpha expression in fusiform PCs is crucial for the dendritic regression and progression of the following step of extension of dendritic processes. However, it does not seem to participate to the last stage of dendritic growth. This study identifies RORalpha as a nuclear receptor crucial for the control of dendritic remodeling during development.
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.4636-05.2006