Inverted Regulation of Multidrug Efflux Pumps, Acid Resistance, and Porins in Benzoate-Evolved Escherichia coli K-12

• Published in: Applied and environmental microbiology 2019-08, Vol.85 (16)
• Main Authors: Moore, Jeremy P, Li, Haofan, Engmann, Morgan L, Bischof, Katarina M, Kunka, Karina S, Harris, Mary E, Tancredi, Anna C, Ditmars, Frederick S, Basting, Preston J, George, Nadja S, Bhagwat, Arvind A, Slonczewski, Joan L
• Format: Article
• Language: English
• Subjects: Acid resistance; Acids; Adenine; Adenine phosphoribosyltransferase; Adenosine; Adenosine deaminase; Antibiotics; Bacteria; Benzoates; Benzoic acid; Biological evolution; Biosynthesis; Catabolism; Chloramphenicol; Chloromycetin; DNA-directed RNA polymerase; E coli; Efflux; Escherichia coli; Fitness; Gene deletion; Gene expression; Genes; Genetics and Molecular Biology; Hydrogenase; Kinases; Lipopolysaccharides; Mutation; Nitrate reductase; Nutrient cycles; Nutrients; Phosphoribosyltransferase; Porins; Post-transcription; Protonmotive force; Pumps; Reductases; Regulation; Reproductive fitness; RNA polymerase; Salicylic acid; Sensitivity; Substrates; Tyrosine
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.00966-19

Benzoic acid, a partial uncoupler of the proton motive force (PMF), selects for sensitivity to chloramphenicol and tetracycline during the experimental evolution of K-12. Transcriptomes of isolates evolved with benzoate showed the reversal of benzoate-dependent regulation, including the downregulation of multidrug efflux pump genes, the gene for the Gad acid resistance regulon, the nitrate reductase genes , and the gene for the acid-consuming hydrogenase Hyd-3. However, the benzoate-evolved strains had increased expression of OmpF and other large-hole porins that admit fermentable substrates and antibiotics. Candidate genes identified from benzoate-evolved strains were tested for their roles in benzoate tolerance and in chloramphenicol sensitivity. Benzoate or salicylate tolerance was increased by deletion of the Gad activator or of the acid fitness island from to the end of the gene encoding Gad regulators and the multidrug pump genes Benzoate tolerance was also increased by deletion of multidrug component gene , RpoS posttranscriptional regulator gene , adenosine deaminase gene , hydrogenase gene (Hyd-3), and the RNA chaperone/DNA-binding regulator gene Chloramphenicol resistance was decreased by mutations in genes for global regulators, such as RNA polymerase alpha subunit gene , the Mar activator gene , and Deletion of lipopolysaccharide biosynthetic kinase gene decreased the rate of growth in chloramphenicol. Isolates from experimental evolution with benzoate had many mutations affecting aromatic biosynthesis and catabolism, such as (encoding tyrosine biosynthesis) and (encoding adenine phosphoribosyltransferase). Overall, benzoate or salicylate exposure selects for the loss of multidrug efflux pumps and of hydrogenases that generate a futile cycle of PMF and upregulates porins that admit fermentable nutrients and antibiotics. Benzoic acid is a common food preservative, and salicylic acid (2-hydroxybenzoic acid) is the active form of aspirin. At high concentrations, benzoic acid conducts a proton across the membrane, depleting the proton motive force. In the absence of antibiotics, benzoate exposure selects against proton-driven multidrug efflux pumps and upregulates porins that admit fermentable substrates but that also allow the entry of antibiotics. Thus, evolution with benzoate and related molecules, such as salicylates, requires a trade-off for antibiotic sensitivity, a trade-off that could help define a stable gut microbiome. Benzoate and salicy