Shewanella decolorationis LDS1 Chromate Resistance

• Published in: Applied and environmental microbiology 2019-09, Vol.85 (18)
• Main Authors: Lemaire, Olivier N, Honoré, Flora A, Tempel, Sébastien, Fortier, Emma M, Leimkühler, Silke, Méjean, Vincent, Iobbi-Nivol, Chantal
• Format: Article
• Language: English
• Subjects: Abiotic stress; Anthraquinone; Anthraquinone dyes; Azo dyes; Biochemistry, Molecular Biology; Bioremediation; Chromate; Chromates; Decoloring; Deoxyribonucleic acid; DNA; Dyes; Environmental Microbiology; Genetic diversity; Genomes; High temperature; Life Sciences; Metabolism; Nucleotide sequence; Phosphorothioate; rRNA 16S; Sediments; Shewanella; Stationary phase; Strain; Stresses; Triarylmethane
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.00777-19

The genus is well known for its genetic diversity, its outstanding respiratory capacity, and its high potential for bioremediation. Here, a novel strain isolated from sediments of the Indian Ocean was characterized. A 16S rRNA analysis indicated that it belongs to the species It was named LDS1. This strain presented an unusual ability to grow efficiently at temperatures from 24°C to 40°C without apparent modifications of its metabolism, as shown by testing respiratory activities or carbon assimilation, and in a wide range of salt concentrations. Moreover, LDS1 tolerates high chromate concentrations. Indeed, it was able to grow in the presence of 4 mM chromate at 28°C and 3 mM chromate at 40°C. Interestingly, whatever the temperature, when the culture reached the stationary phase, the strain reduced the chromate present in the growth medium. In addition, LDS1 degrades different toxic dyes, including anthraquinone, triarylmethane, and azo dyes. Thus, compared to , this strain presented better capacity to cope with various abiotic stresses, particularly at high temperatures. The analysis of genome sequence preliminary data indicated that, in contrast to and S12, LDS1 possesses the phosphorothioate modification machinery that has been described as participating in survival against various abiotic stresses by protecting DNA. We demonstrate that its heterologous production in allows it to resist higher concentrations of chromate. species have long been described as interesting microorganisms in regard to their ability to reduce many organic and inorganic compounds, including metals. However, members of the genus are often depicted as cold-water microorganisms, although their optimal growth temperature usually ranges from 25 to 28°C under laboratory growth conditions. LDS1 is highly attractive, since its metabolism allows it to develop efficiently at temperatures from 24 to 40°C, conserving its ability to respire alternative substrates and to reduce toxic compounds such as chromate or toxic dyes. Our results clearly indicate that this novel strain has the potential to be a powerful tool for bioremediation and unveil one of the mechanisms involved in its chromate resistance.