Draft genome sequence of Solanum aethiopicum provides insights into disease resistance, drought tolerance, and the evolution of the genome

• Published in: Gigascience 2019-10, Vol.8 (10)
• Main Authors: Song, Bo, Song, Yue, Fu, Yuan, Kizito, Elizabeth Balyejusa, Kamenya, Sandra Ndagire, Kabod, Pamela Nahamya, Liu, Huan, Muthemba, Samuel, Kariba, Robert, Njuguna, Joyce, Maina, Solomon, Stomeo, Francesca, Djikeng, Appolinaire, Hendre, Prasad S, Chen, Xiaoli, Chen, Wenbin, Li, Xiuli, Sun, Wenjing, Wang, Sibo, Cheng, Shifeng, Muchugi, Alice, Jamnadass, Ramni, Shapiro, Howard-Yana, Van Deynze, Allen, Yang, Huanming, Wang, Jian, Xu, Xun, Odeny, Damaris Achieng, Liu, Xin
• Format: Article
• Language: English
• Subjects: Acclimatization - genetics; Breeding; Crop diseases; Crops; Disease resistance; Disease Resistance - genetics; Domestication; Drought resistance; Droughts; Evolution, Molecular; Genes; Genome, Plant; Genomes; Long terminal repeat; Nucleotide sequence; Nucleotides; Phylogeny; Polymorphism, Single Nucleotide; Proteins; Retroelements; Single-nucleotide polymorphism; Solanaceae; Solanum - genetics; Solanum aethiopicum; Solanum melongena; Terminal Repeat Sequences; Vegetables
• ISSN: 2047-217X; 2047-217X
• DOI: 10.1093/gigascience/giz115

Abstract Background The African eggplant (Solanum aethiopicum) is a nutritious traditional vegetable used in many African countries, including Uganda and Nigeria. It is thought to have been domesticated in Africa from its wild relative, Solanum anguivi. S. aethiopicum has been routinely used as a source of disease resistance genes for several Solanaceae crops, including Solanum melongena. A lack of genomic resources has meant that breeding of S. aethiopicum has lagged behind other vegetable crops. Results We assembled a 1.02-Gb draft genome of S. aethiopicum, which contained predominantly repetitive sequences (78.9%). We annotated 37,681 gene models, including 34,906 protein-coding genes. Expansion of disease resistance genes was observed via 2 rounds of amplification of long terminal repeat retrotransposons, which may have occurred ∼1.25 and 3.5 million years ago, respectively. By resequencing 65 S. aethiopicum and S. anguivi genotypes, 18,614,838 single-nucleotide polymorphisms were identified, of which 34,171 were located within disease resistance genes. Analysis of domestication and demographic history revealed active selection for genes involved in drought tolerance in both “Gilo” and “Shum” groups. A pan-genome of S. aethiopicum was assembled, containing 51,351 protein-coding genes; 7,069 of these genes were missing from the reference genome. Conclusions The genome sequence of S. aethiopicum enhances our understanding of its biotic and abiotic resistance. The single-nucleotide polymorphisms identified are immediately available for use by breeders. The information provided here will accelerate selection and breeding of the African eggplant, as well as other crops within the Solanaceae family.