Taurine-mediated browning of white adipose tissue is involved in its anti-obesity effect in mice

• Published in: The Journal of biological chemistry 2019-10, Vol.294 (41), p.15014-15024
• Main Authors: Guo, Ying-Ying, Li, Bai-Yu, Peng, Wan-Qiu, Guo, Liang, Tang, Qi-Qun
• Format: Article
• Language: English
• Subjects: adaptive thermogenesis; Adipocytes - drug effects; Adipocytes - pathology; adipose tissue; Adipose Tissue, Brown - drug effects; Adipose Tissue, Brown - pathology; Adipose Tissue, White - drug effects; Adipose Tissue, White - pathology; AMP-activated kinase (AMPK); AMP-Activated Protein Kinases - metabolism; Animals; Anti-Obesity Agents - pharmacology; Anti-Obesity Agents - therapeutic use; browning of adipose tissue; Cell Biology; energy metabolism; Energy Metabolism - drug effects; Gene Expression Regulation - drug effects; Insulin Resistance; metabolic regulation; Mice; obesity; Obesity - drug therapy; Obesity - metabolism; Obesity - pathology; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) (PPARGC1A); Signal Transduction - drug effects; taurine; Taurine - pharmacology; Taurine - therapeutic use; Thermogenesis - drug effects; UCP1
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.RA119.009936

Taurine, a nonprotein amino acid, is widely distributed in almost all animal tissues. Ingestion of taurine helps to improve obesity and its related metabolic disorders. However, the molecular mechanism underlying the protective role of taurine against obesity is not completely understood. In this study, it was found that intraperitoneal treatment of mice with taurine alleviated high-fat diet (HFD)-induced obesity, improved insulin sensitivity, and increased energy expenditure and adaptive thermogenesis of the mice. Meanwhile, administration of the mice with taurine markedly induced the browning of inguinal white adipose tissue (iWAT) with significantly elevated expression of PGC1α, UCP1, and other thermogenic genes in iWAT. In vitro studies indicated that taurine also induced the development of brown-like adipocytes in C3H10T1/2 white adipocytes. Knockdown of PGC1α blunted the role of taurine in promoting the brown-like adipocyte phenotypes in C3H10T1/2 cells. Moreover, taurine treatment enhanced AMPK phosphorylation in vitro and in vivo, and knockdown of AMPKα1 prevented taurine-mediated induction of PGC1α in C3H10T1/2 cells. Consistently, specific knockdown of PGC1α in iWAT of the HFD-fed mice inhibited taurine-induced browning of iWAT, with the role of taurine in the enhancement of adaptive thermogenesis, the prevention of obesity, and the improvement of insulin sensitivity being partially impaired. These results reveal a functional role of taurine in facilitating the browning of white adipose tissue, which depends on the induction of PGC1α. Our studies also suggest a potential mechanism for the protective role of taurine against obesity, which involves taurine-mediated browning of white adipose tissue.