The Localization of the Brain-Specific Inorganic Phosphate Transporter Suggests a Specific Presynaptic Role in Glutamatergic Transmission

Molecular cloning has recently identified a vertebrate brain-specific Na+-dependent inorganic phosphate transporter (BNPI). BNPI has strong sequence similarity to EAT-4, a Caenorhabditis elegans protein implicated in glutamatergic transmission. To characterize the physiological role of BNPI, we have...

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Bibliographic Details
Published in:The Journal of neuroscience 1998-11, Vol.18 (21), p.8648-8659
Main Authors: Bellocchio, Elizabeth E, Hu, Hailan, Pohorille, Alicia, Chan, June, Pickel, Virginia M, Edwards, Robert H
Format: Article
Language:English
Subjects:
Animals
Brain - metabolism
Brain - ultrastructure
Carrier Proteins - metabolism
Carrier Proteins - physiology
Glutamic Acid - metabolism
Hippocampus - chemistry
Hippocampus - metabolism
Immunohistochemistry
Male
Microscopy, Electron
Presynaptic Terminals - metabolism
Presynaptic Terminals - ultrastructure
Rats
Rats, Sprague-Dawley
Sodium-Phosphate Cotransporter Proteins
Symporters
Synaptic Vesicles - metabolism
Synaptic Vesicles - ultrastructure
Synaptophysin - analysis
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Summary:Molecular cloning has recently identified a vertebrate brain-specific Na+-dependent inorganic phosphate transporter (BNPI). BNPI has strong sequence similarity to EAT-4, a Caenorhabditis elegans protein implicated in glutamatergic transmission. To characterize the physiological role of BNPI, we have generated an antibody to the protein. Immunocytochemistry of rat brain sections shows a light microscopic pattern that is suggestive of reactivity in nerve terminals. Excitatory projections are labeled prominently, and ultrastructural analysis confirms that BNPI localizes almost exclusively to terminals forming asymmetric excitatory-type synapses. Although BNPI depends on a Na+ gradient and presumably functions at the plasma membrane, both electron microscopy and biochemical fractionation show that BNPI associates preferentially with the membranes of small synaptic vesicles. The results provide anatomic evidence of a specific presynaptic role for BNPI in glutamatergic neurotransmission, consistent with the phenotype of eat-4 mutants. Because an enzyme known as the phosphate-activated glutaminase produces glutamate for release as a neurotransmitter, BNPI may augment excitatory transmission by increasing cytoplasmic phosphate concentrations within the nerve terminal and hence increasing glutamate synthesis. Expression of BNPI on synaptic vesicles suggests a mechanism for neural activity to regulate the function of BNPI.
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.18-21-08648.1998