Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-point accumulation for imaging in nanoscale topography

In the brain, the strength of each individual synapse is defined by the complement of proteins present or the “local proteome.” Activity-dependent changes in synaptic strength are the result of changes in this local proteome and posttranslational protein modifications. Although most synaptic protein...

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Bibliographic Details
Published in:Neurophotonics (Print) 2019-07, Vol.6 (3), p.035008-035008
Main Authors: Böger, Carolin, Hafner, Anne-Sophie, Schlichthärle, Thomas, Strauss, Maximilian T, Malkusch, Sebastian, Endesfelder, Ulrike, Jungmann, Ralf, Schuman, Erin M, Heilemann, Mike
Format: Article
Language:English
Subjects:
Antibodies
Binding sites
Brain architecture
Calibration
Hippocampus
Localization
Microscopy
Neuroimaging
Neurons
Photobleaching
Proteins
Proteomes
Research Papers
Spatial discrimination
Synaptic strength
Topography
α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
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Summary:In the brain, the strength of each individual synapse is defined by the complement of proteins present or the “local proteome.” Activity-dependent changes in synaptic strength are the result of changes in this local proteome and posttranslational protein modifications. Although most synaptic proteins have been identified, we still know little about protein copy numbers in individual synapses and variations between synapses. We use DNA-point accumulation for imaging in nanoscale topography as a single-molecule super-resolution imaging technique to visualize and quantify protein copy numbers in single synapses. The imaging technique provides near-molecular spatial resolution, is unaffected by photobleaching, enables imaging of large field of views, and provides quantitative molecular information. We demonstrate these benefits by accessing copy numbers of surface AMPA-type receptors at single synapses of rat hippocampal neurons along dendritic segments.
ISSN:2329-423X
2329-4248
DOI:10.1117/1.NPh.6.3.035008