Occurrence of Bacterial Markers and Antibiotic Resistance Genes in Sub-Saharan Rivers Receiving Animal Farm Wastewaters

Antibiotic resistant bacteria and genes which confer resistance to antibiotics from human/animal sources are currently considered a serious environmental and a public health concern. This problem is still little investigated in aquatic environment of developing countries according to the different c...

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Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.14847-10, Article 14847
Main Authors: Al Salah, Dhafer Mohammed M., Laffite, Amandine, Poté, John
Format: Article
Language:English
Subjects:
101/1
38/22
38/77
704/172
704/242
Agricultural wastes
Animals
Anthropogenic factors
Antibiotic resistance
Antibiotics
Aquatic ecosystems
Aquatic environment
Bacteria
Cities
Climatic conditions
Contaminants
Democratic Republic of the Congo
Developing Countries
Drug resistance
Drug Resistance, Microbial
E coli
Enterococcus
Enterococcus - genetics
Enterococcus - isolation & purification
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
Farms
Genes, Bacterial
Humanities and Social Sciences
Humans
LDCs
Microbial contamination
multidisciplinary
Polymerase chain reaction
Pseudomonas
Pseudomonas - genetics
Pseudomonas - isolation & purification
Public health
Rivers
Rivers - microbiology
rRNA 16S
Science
Science (multidisciplinary)
Sediments
Swine
Wastewater - microbiology
Water Microbiology
Water Quality
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Summary:Antibiotic resistant bacteria and genes which confer resistance to antibiotics from human/animal sources are currently considered a serious environmental and a public health concern. This problem is still little investigated in aquatic environment of developing countries according to the different climatic conditions. In this research, the total bacterial load, the abundance of relevant bacteria ( Escherichia coli (E . coli) , Enterococcus (Ent), and Pseudomonas ), and antibiotic resistance genes (ARGs: bla OXA-48 , bla CTX-M , sul1 , sul2 , sul3 , and tet(B) ) were quantified using Quantitative Polymerase Chain Reaction (qPCR) in sediments from two rivers receiving animal farming wastewaters under tropical conditions in Kinshasa, capital city of the Democratic Republic of the Congo. Human and pig host-specific markers were exploited to examine the sources of contamination. The total bacterial load correlated with relevant bacteria and genes bla OXA-48 , sul3 , and tet(B) (P value 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-51421-4