Image analysis systems for the detection of disseminated breast cancer cells on bone-marrow cytospins

We assessed the accuracy of automated cell imaging systems when compared to manual evaluation of cytospin slides in determining the presence of cytokeratin‐positive, disseminated breast cancer cells in bone marrow aspirates. A total of 298 cytospin slides of bone marrow aspirates were first evaluate...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2005, Vol.19 (3), p.115-119
Main Authors: Becker, Sven, Becker-Pergola, Graziella, Fehm, Tanja, Emig, Robert, Wallwiener, Diethelm, Solomayer, Erich-Franz
Format: Article
Language:English
Subjects:
Biopsy, Needle
Bone Marrow - pathology
Bone Marrow Neoplasms - pathology
Bone Marrow Neoplasms - secondary
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Female
Humans
Image Processing, Computer-Assisted - instrumentation
immunocytology
immunohistochemistry
micrometastases
minimal residual disease
occult tumor cells
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Summary:We assessed the accuracy of automated cell imaging systems when compared to manual evaluation of cytospin slides in determining the presence of cytokeratin‐positive, disseminated breast cancer cells in bone marrow aspirates. A total of 298 cytospin slides of bone marrow aspirates were first evaluated by individual screening by one expert immunocytologist. Subsequently, all 298 slides were evaluated by the Automated Cell Imaging System (ACIS) by ChromaVision™. Two separate analysis cycles were performed using ACIS. The results of the two ACIS analysis cycles were almost identical: in 293 out of 298 samples (98.3%), identical numbers of disseminated breast cancer cells were detected. In the remaining five samples (1.7%), the result of the two ACIS analysis cycles differed by only one tumor cell. By using the manual technique, 120 cytospin samples were found to be positive. ACIS was able to detect additional tumor cells in 64 cases. Not once did ACIS miss tumor cells when compared to the manual technique. Using ACIS, we were able to determine the bone marrow status of patients with nonmetastatic breast cancer faster, with greater accuracy, and with greater reproducibility than with the manual technique. J. Clin. Lab. Anal. 19:115–119, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.20064