N‐acetyl transferase 2/environmental factors and their association as a modulating risk factor for sporadic colon and rectal cancer

• Published in: Journal of clinical laboratory analysis 2017-09, Vol.31 (5), p.e22098-n/a
• Main Authors: Procopciuc, Lucia M., Osian, Gelu, Iancu, Mihaela
• Format: Article
• Language: English
• Subjects: Alcohol; alcohol consumption; Alcohol Drinking; Alleles; Arylamine N-Acetyltransferase - genetics; Case-Control Studies; Colon cancer; Colonic Neoplasms - epidemiology; Colonic Neoplasms - genetics; Colorectal cancer; Cooking; Environmental factors; Feeding Behavior; Female; fried red meat intake; genetic analysis; Genotypes; Health risk assessment; Humans; Male; Meat; Middle Aged; NAT2 rapid acetylators; Polymerase chain reaction; Rectal Neoplasms - epidemiology; Rectal Neoplasms - genetics; Rectum; Restriction fragment length polymorphism; Risk Factors; Smoking; smoking behavior
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.22098

Objectives The aim of this study was to evaluate the association between environmental factors and colon or rectal cancer after adjusting for N‐acetyl transferase 2 (NAT2) phenotypes. Methods Ninety‐six patients with sporadic colon cancer, 54 with sporadic rectal cancer and 162 control subjects were genotyped for NAT2‐T341C, G590A, G857A, A845C, and C481T using sequencing and PCR‐RFLP analysis. Results The risk for colon cancer was increased in carriers of the homozygous negative genotypes for NAT2*5C‐T341C, NAT2*6B‐G590A, NAT2*7B‐G857A, NAT2*18‐A845C, and NAT2*5A‐C481T. The risk for rectal cancer was increased in carriers of the homozygous negative genotypes for NAT2*5C‐T341C, NAT2*7B‐G857A, and NAT2*5A‐C481T. High fried red meat intake associated with NAT2‐T341C, G590A, G857A, A845C, and C481T rapid acetylator allele determines a risk of 2.39 (P=.002), 2.39 (P=.002), 2.37 (P=.002), 2.28 (P=.004), and 2.51 (P=.001), respectively, for colon cancer, whereas in the case of rectal cancer, the risk increased to 7.55 (P