Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

A number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize H uman M ilk O ligosaccharides (HMOs). In the curr...

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Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.15427-20, Article 15427
Main Authors: James, Kieran, Bottacini, Francesca, Contreras, Jose Ivan Serrano, Vigoureux, Mariane, Egan, Muireann, Motherway, Mary O’connell, Holmes, Elaine, van Sinderen, Douwe
Format: Article
Language:English
Subjects:
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631/326/41/2530
631/326/41/88
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82/83
Astrophysics
Baby foods
Bifidobacterium - metabolism
Breast Feeding
Breast milk
Breastfeeding & lactation
Feces - microbiology
Female
Fucose
Gastrointestinal Microbiome
Genomes
Humanities and Social Sciences
Humans
Infant, Newborn
Infants
Male
Metabolic pathways
Metabolism
Metabolites
Milk, Human - metabolism
multidisciplinary
Niches
Nucleotide sequence
Oligosaccharides
Oligosaccharides - metabolism
Physics
Science
Science (multidisciplinary)
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Summary:A number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize H uman M ilk O ligosaccharides (HMOs). In the current study, we isolated a novel strain of Bifidobacterium kashiwanohense , named APCKJ1, from the faeces of a four-week old breastfed infant, based on the ability of the strain to utilise the HMO component fucosyllactose. We then determined the full genome sequence of this strain, and employed the generated data to analyze fucosyllactose metabolism in B. kashiwanohense APCKJ1. Transcriptomic and growth analyses, combined with metabolite analysis, in vitro hydrolysis assays and heterologous expression, allowed us to elucidate the pathway for fucosyllactose metabolism in B. kashiwanohense APCKJ1. Homologs of the key genes for this metabolic pathway were identified in particular in infant-derived members of the Bifdobacterium genus, revealing the apparent niche-specific nature of this pathway, and allowing a broad perspective on bifidobacterial fucosyllactose and L-fucose metabolism.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-51901-7