Assessment of Haemonchus contortus larval and adult somatic antigens in sero-diagnosis of haemonchosis in naturally infected sheep and goats

• Published in: Journal of parasitic diseases 2019-12, Vol.43 (4), p.718-725
• Main Authors: Hassan, Noha M. F., Aboelsoued, Dina, Farag, Tarek K., Hassan, Soad E., Abu El Ezz, Nadia M. T.
• Format: Article
• Language: English
• Subjects: Antigens; Diagnosis; Haemonchus contortus; Health Promotion and Disease Prevention; Infectious Diseases; Intestinal parasites; Medicine; Medicine & Public Health; Original; Original Article; Sheep
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-019-01152-0

The current work was carried out to evaluate the potency of larval and adult somatic Haemonchus contortus ( H. contortus ) antigens in detection of haemonchosis among sheep and goats using ELISA. Two hundred and forty-three fecal and blood samples were randomly collected from small ruminants (107 sheep and 136 goats) in Beni-Suef Governorate, Egypt, during the period from June to August 2018. The fecal analysis exhibited that 26.33% of the small ruminants were infected with gastrointestinal nematodes. The overall prevalence of H. contortus was reached 22.22% whereas it was 27.10% and 18.38% among sheep and goats, respectively. The current study elucidated that the larval antigen has claimed more superior diagnostic results compared to the adult somatic H. contortus antigen. The apparent overall sero-prevalence among small ruminants was reached 51.85%. Separately, it was 64.48% in sheep and 41.91% in goats. The larval antigen had proved 96.55% sensitivity and 47.43% specificity, for sheep serum samples. Meanwhile, sensitivity and specificity for goats’ sera were 100% and 71.17%, respectively. Diagnostic efficacy of ELISA was recorded 60.74% in sheep and 76.47% in goats. This study deduced that the larval antigen has proved the priority and the potency for diagnosis of H. contortus infection. Moreover, haemonchosis is a prevalent disease among the examined sheep and goats.