Metal-enhanced fluorescence: an emerging tool in biotechnology

Over the past 15 years, fluorescence has become the dominant detection/sensing technology in medical diagnostics and biotechnology. Although fluorescence is a highly sensitive technique, where single molecules can readily be detected, there is still a drive for reduced detection limits. The detectio...

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Bibliographic Details
Published in:Current opinion in biotechnology 2005-02, Vol.16 (1), p.55-62
Main Authors: Aslan, Kadir, Gryczynski, Ignacy, Malicka, Joanna, Matveeva, Evgenia, Lakowicz, Joseph R, Geddes, Chris D
Format: Article
Language:English
Subjects:
Biotechnology - methods
Biotechnology - trends
Fluorescence
Fluorescent Dyes
fluorophores
In Situ Hybridization, Fluorescence - methods
In Situ Hybridization, Fluorescence - trends
Metals - chemistry
Microscopy, Fluorescence - methods
Microscopy, Fluorescence - trends
Nanotubes - chemistry
Nanotubes - ultrastructure
Q1
Radiation
Reviews
Spectrometry, Fluorescence - methods
Spectrometry, Fluorescence - trends
Surface Plasmon Resonance - methods
Surface Plasmon Resonance - trends
Technology Assessment, Biomedical
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Summary:Over the past 15 years, fluorescence has become the dominant detection/sensing technology in medical diagnostics and biotechnology. Although fluorescence is a highly sensitive technique, where single molecules can readily be detected, there is still a drive for reduced detection limits. The detection of a fluorophore is usually limited by its quantum yield, autofluorescence of the samples and/or the photostability of the fluorophores; however, there has been a recent explosion in the use of metallic nanostructures to favorably modify the spectral properties of fluorophores and to alleviate some of these fluorophore photophysical constraints. The use of fluorophore–metal interactions has been termed radiative decay engineering, metal-enhanced fluorescence or surface-enhanced fluorescence.
ISSN:0958-1669
1879-0429
DOI:10.1016/j.copbio.2005.01.001