Escherichia coli and Salmonella Enteritidis dual-species biofilms: interspecies interactions and antibiofilm efficacy of phages

• Published in: Scientific reports 2019-12, Vol.9 (1), p.18183, Article 18183
• Main Authors: Milho, C., Silva, Maria Daniela, Alves, Diana Filipa Barros, Oliveira, Hugo Alexandre Mendes, Sousa, Clara, Pastrana, Lorenzo M., Azeredo, Joana, Sillankorva, Sanna
• Format: Article
• Language: English
• Subjects: 631/158; 631/1647; 631/326; 631/61; Antimicrobial resistance; Biofilms; Confocal microscopy; E coli; Escherichia coli; Foodborne pathogens; Humanities and Social Sciences; Infrared spectroscopy; multidisciplinary; New combinations; Pathogens; Phages; Salmonella; Salmonella Enteritidis; Science; Science & Technology; Science (multidisciplinary); Spatial distribution; Species; Spectrum analysis; Strains (organisms); Virulence
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-54847-y

Escherichia coli and Salmonella Enteritidis are foodborne pathogens forming challenging biofilms that contribute to their virulence, antimicrobial resistance, and survival on surfaces. Interspecies interactions occur between species in mixed biofilms promoting different outcomes to each species. Here we describe the interactions between E. coli and S. Enteritidis strains, and their control using specific phages. Single-species biofilms presented more cells compared to dual-species biofilms. The spatial organization of strains, observed by confocal microscopy, revealed similar arrangements in both single- and dual-species biofilms. The EPS matrix composition, assessed by Fourier-transform infrared spectroscopy, disclosed that the spectra extracted from the different dual-species biofilms can either be a combination of both species EPS matrix components or that the EPS matrix of one species predominates. Phages damaged more the single-species biofilms than the mixed biofilms, showing also that the killing efficacy was greatly dependent on the phage growth characteristics, bacterial growth parameters, and bacterial spatial distribution in biofilms. This combination of methodologies provides new knowledge of species-species and phage-host interactions in biofilms of these two major foodborne pathogens. This project also received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreements No 713640, and No. 778388. This work was supported by the Portuguese Foundation for Science and Technology under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. CM and MDS acknowledge the Portuguese Foundation for Science and Technology (FCT) for their grants SFRH/BD/94434/2013 and SFRH/BD/128825/2017. The pNUT086 plasmid was kindly provided by Dr. Knut Drescher from Max Planck Institute for Terrestrial Microbiology, Germany.