Comparison of Escherichia coli surface attachment methods for single-cell microscopy

For in vivo , single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial ph...

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Bibliographic Details
Published in:Scientific reports 2019-12, Vol.9 (1), p.19418-11, Article 19418
Main Authors: Wang, Yao-Kuan, Krasnopeeva, Ekaterina, Lin, Ssu-Yuan, Bai, Fan, Pilizota, Teuta, Lo, Chien-Jung
Format: Article
Language:English
Subjects:
631/1647
631/326
631/57
Bacteria
Bacterial Adhesion
Cells, Immobilized - cytology
E coli
Escherichia coli
Escherichia coli - cytology
Escherichia coli - growth & development
Growth rate
Humanities and Social Sciences
Hydrogen-Ion Concentration
Light microscopy
Microscopy
Microscopy - methods
multidisciplinary
pH effects
Physiology
Science
Science (multidisciplinary)
Single-Cell Analysis
Surface Properties
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Summary:For in vivo , single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli , and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-55798-0