From Local to Global Modeling for Characterizing Calcium Dynamics and Their Effects on Electrical Activity and Exocytosis in Excitable Cells

• Published in: International journal of molecular sciences 2019-11, Vol.20 (23), p.6057
• Main Authors: Montefusco, Francesco, Pedersen, Morten G
• Format: Article
• Language: English
• Subjects: Action Potentials - physiology; Animals; Approximation; Bursting; Calcium - metabolism; Calcium channels; Calcium channels (voltage-gated); Calcium Channels - metabolism; Calcium influx; Calcium ions; Calcium signalling; Computer Simulation; Cytoplasmic Granules - chemistry; Cytoplasmic Granules - metabolism; Exocytosis; Exocytosis - physiology; Firing pattern; Granular materials; Hormones; Humans; Ion Channel Gating - physiology; Ion channels; Kinetics; Large-Conductance Calcium-Activated Potassium Channels - metabolism; Models, Biological; Normalizing; Pituitary; Pituitary Gland - cytology; Pituitary Gland - metabolism; Potassium conductance; Potassium currents; Probability; Proteins; Resistance; Somatotrophs - cytology; Somatotrophs - metabolism; Stoichiometry
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20236057

Electrical activity in neurons and other excitable cells is a result of complex interactions between the system of ion channels, involving both global coupling (e.g., via voltage or bulk cytosolic Ca concentration) of the channels, and local coupling in ion channel complexes (e.g., via local Ca concentration surrounding Ca channels (CaVs), the so-called Ca nanodomains). We recently devised a model of large-conductance BK potassium currents, and hence BK -CaV complexes controlled locally by CaVs via Ca nanodomains. We showed how different CaV types and BK -CaV stoichiometries affect whole-cell electrical behavior. Ca nanodomains are also important for triggering exocytosis of hormone-containing granules, and in this regard, we implemented a strategy to characterize the local interactions between granules and CaVs. In this study, we coupled electrical and exocytosis models respecting the local effects via Ca nanodomains. By simulating scenarios with BK -CaV complexes with different stoichiometries in pituitary cells, we achieved two main electrophysiological responses (continuous spiking or bursting) and investigated their effects on the downstream exocytosis process. By varying the number and distance of CaVs coupled with the granules, we found that bursting promotes exocytosis with faster rates than spiking. However, by normalizing to Ca influx, we found that bursting is only slightly more efficient than spiking when CaVs are far away from granules, whereas no difference in efficiency between bursting and spiking is observed with close granule-CaV coupling.