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The Characteristics and Expression Profile of Transferrin in the Accessory Nidamental Gland of the Bigfin Reef Squid during Bacteria Transmission
The accessory nidamental gland (ANG) is a female reproductive organ found in most squid and cuttlefish that contains a consortium of bacteria. These symbiotic bacteria are transmitted from the marine environment and selected by the host through an unknown mechanism. In animals, a common antimicrobia...
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Published in: | Scientific reports 2019-12, Vol.9 (1), p.20163-11, Article 20163 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The accessory nidamental gland (ANG) is a female reproductive organ found in most squid and cuttlefish that contains a consortium of bacteria. These symbiotic bacteria are transmitted from the marine environment and selected by the host through an unknown mechanism. In animals, a common antimicrobial mechanism of innate immunity is iron sequestration, which is based on the development of transferrin (TF)-like proteins. To understand this mechanism of host-microbe interaction, we attempted to characterize the role of transferrin in bigfin reef squid (
Sepioteuthis lessoniana
) during bacterial transmission. qPCR analysis showed that
Tf
was exclusively expressed in the outer layer of ANG,and this was confirmed by
in situ
hybridization, which showed that
Tf
was localized in the outer epithelial cell layer of the ANG. Western blot analysis indicated that TF is a soluble glycoprotein. Immunohistochemical staining also showed that TF is localized in the outer epithelial cell layer of the ANG and that it is mainly expressed in the outer layer during ANG growth. These results suggest that robust
Tf
mRNA and TF protein expression in the outer layer of the ANG plays an important role in microbe selection by the host during bacterial transmission. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-019-56584-8 |