The Occurrence of Multidrug Resistant Bacteria in the Urine of Healthy Dogs and Dogs with Cystitis

• Published in: Animals (Basel) 2019-12, Vol.9 (12), p.1087
• Main Authors: Yamanaka, Andreia R, Hayakawa, Alessandra T, Rocha, Ícaro S M, Dutra, Valéria, Souza, Valeria R F, Cruz, José N, Camargo, Lázaro M, Nakazato, Luciano
• Format: Article
• Language: English
• Subjects: Acids; Ampicillin; Animals; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bacteria; Confidence intervals; Cystitis; Diagnosis; Disease transmission; Distribution; Dog diseases; Dogs; Drug resistance; E coli; Evaluation; Health care; Imipenem; Impact resistance; Meropenem; Methods; Microbial drug resistance; Microorganisms; Multidrug resistance; Penicillins; Pets; Public health; Public health movements; Quinolones; Statistical analysis; Tetracyclines; Urinalysis; Urinary tract; Urinary tract infections; Urine; World health
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani9121087

The objectives of this study were to evaluate the occurrence of microorganisms, the antimicrobial susceptibility profile, and the presence of multidrug resistant (MDR) bacteria in the urine of clinically healthy dogs and dogs with cystitis. The urine was collected through cystocentesis. Subsequently, culture and antimicrobial susceptibility tests were performed. The isolates were classified based on their resistance profile, to evaluate the presence of MDR bacteria. Statistical analyses were performed using the chi-squared or Fisher's exact tests. Bacterial isolates were present in 24.39% of the dogs in the control group, and 60.27% of the dogs in the cystitis group. The cystitis group was associated with a higher risk of bacterial isolates than the control group (odds ratio = 7.5; 95% confidence interval = 2.81-22.40). The main isolates were spp., , spp., and spp. in both groups. A high percentage of isolates were resistant to ampicillin in both groups. The lowest resistance presented by the isolates in both groups was to meropenem. Only the resistance to quinolones was different between the groups. The proportions of MDR isolates were 70% (7/10) and 65.91% (29/44) in the control and cystitis groups, respectively. The results showed the presence of MDR bacteria in the urine of both the healthy dogs, and the dogs with cystitis. The drug resistance was high, reinforcing the importance of establishing an effective treatment approach against urinary tract infections in pets, to minimize the spread of bacterial resistance and its impact on public health.