Preparation of Squalene Oil-Based Emulsion Adjuvants Employing a Self-Emulsifying Drug Delivery System and Assessment of Mycoplasma hyopneumoniae -Specific Antibody Titers in BALB/c Mice

In this study, a self-emulsifying drug delivery system (SEDDS) was employed to prepare novel squalene oil-based emulsion adjuvants. Deionized water, 0.01% and 0.02% ( / ) carbomer solutions of C-971P NF and C-940 grades were used to prepare emulsions containing 3%, 5% and 10% of squalene oil. Altoge...

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Bibliographic Details
Published in:Pharmaceutics 2019-12, Vol.11 (12), p.667
Main Authors: Bastola, Rakesh, Seo, Jo-Eun, Keum, Taekwang, Noh, Gyubin, Choi, Jae Woong, Shin, Jong Il, Kim, Ju Hun, Lee, Sangkil
Format: Article
Language:English
Subjects:
Animal vaccines
Antibodies
Antigens
Biotechnology
Drug delivery systems
Surfactants
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Summary:In this study, a self-emulsifying drug delivery system (SEDDS) was employed to prepare novel squalene oil-based emulsion adjuvants. Deionized water, 0.01% and 0.02% ( / ) carbomer solutions of C-971P NF and C-940 grades were used to prepare emulsions containing 3%, 5% and 10% of squalene oil. Altogether 15 candidate emulsions were prepared and used as adjuvants for the delivery of a combination vaccine containing a porcine circovirus type 2 (PCV2) antigen and inactivated (J101 strain) antigen. Most of the emulsions showed droplet sizes in the submicron range and maintained zeta potential values between -40 mV to 0 mV for six months, indicating good physical stability as a vaccine adjuvant. Emulsion-based candidate adjuvants prepared with SEDDS technology stimulated IgG, IgG1 and IgG2a like a currently commercially available adjuvant, Montanide ISA 201, and they were safe and their -specific antibody titers were considered as comparable with that of Montanide ISA 201.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics11120667