Biotin proximity tagging favours unfolded proteins and enables the study of intrinsically disordered regions

• Published in: Communications biology 2020-01, Vol.3 (1), p.38, Article 38
• Main Authors: Minde, David-Paul, Ramakrishna, Manasa, Lilley, Kathryn S.
• Format: Article
• Language: English
• Subjects: 101/1; 101/47; 101/58; 631/114; 631/45; 631/535; 631/80; 631/92; 82; Amino Acid Sequence; Biology; Biomedical and Life Sciences; Biotin; Biotin - chemistry; Biotin - metabolism; Biotinylation; Humans; Intrinsically Disordered Proteins - chemistry; Intrinsically Disordered Proteins - metabolism; Life Sciences; Lysine - metabolism; Models, Molecular; Plasticity; Post-translation; Protein Binding; Protein Conformation; Protein folding; Protein Interaction Domains and Motifs; Protein Processing, Post-Translational; Protein structure; Protein Transport; Protein Unfolding; Proteins; Proteomics; Proteomics - methods; Ribosomes; Ribosomes - metabolism; Structure-Activity Relationship; Tyrosine - metabolism
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-020-0758-y

Intrinsically Disordered Regions (IDRs) are enriched in disease-linked proteins known to have multiple post-translational modifications, but there is limited in vivo information about how locally unfolded protein regions contribute to biological functions. We reasoned that IDRs should be more accessible to targeted in vivo biotinylation than ordered protein regions, if they retain their flexibility in human cells. Indeed, we observed increased biotinylation density in predicted IDRs in several cellular compartments >20,000 biotin sites from four proximity proteomics studies. We show that in a biotin ‘painting’ time course experiment, biotinylation events in Escherichia coli ribosomes progress from unfolded and exposed regions at 10 s, to structured and less accessible regions after five minutes. We conclude that biotin proximity tagging favours sites of local disorder in proteins and suggest the possibility of using biotin painting as a method to gain unique insights into in vivo condition-dependent subcellular plasticity of proteins. David-Paul Minde, Manasa Ramakrishna et al. look at intrinsically disordered regions of disease-linked proteins in vivo by biotinylation. They show that biotin “painting” could be used as a method to examine the condition-dependent plasticity of proteins in vivo.