Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway

• Published in: Cell cycle (Georgetown, Tex.) Tex.), 2020-02, Vol.19 (3), p.376-389
• Main Authors: Wang, Lei, Tian, Meilei, Hao, Yugui
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - genetics; Astrocytes - metabolism; Autophagy - genetics; Blood-Brain Barrier - metabolism; Cells, Cultured; Cerebral Hemorrhage - enzymology; Cerebral Hemorrhage - genetics; Cerebral Hemorrhage - metabolism; Cerebral Hemorrhage - pathology; Cytokines - metabolism; Disease Models, Animal; Endothelial Cells - metabolism; Gene Knockdown Techniques; Inflammation - genetics; Inflammation - metabolism; Male; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurons - enzymology; Neurons - metabolism; Neurons - pathology; Rats; Rats, Sprague-Dawley; Receptors, Growth Factor - genetics; Receptors, Growth Factor - metabolism; Research Paper; Signal Transduction - genetics; TOR Serine-Threonine Kinases - metabolism; Up-Regulation
• ISSN: 1538-4101; 1551-4005
• DOI: 10.1080/15384101.2019.1711318

Rupture of weakened blood vessels could lead to severe intracerebral hemorrhage (ICH) and brain injuries. This study was designed to explore the roles of p75 neurotrophin receptor (p75 ) in neuronal autophagy in ICH rats. An ICH rat model was established, and then gain and loss of functions of p75 in rat tissues were performed. Then, the pathologic morphology, water content, and inflammation in brain tissues were assessed. Western blot analysis was applied to detect the levels of inflammatory proteins, apoptosis- and autophagy-related proteins, and the mammalian target of rapamycin (mTOR) pathway-related proteins. Neuronal autophagy was further measured with mTOR activated. experiments were also performed on brain microvascular endothelial cells (BMECs) and astrocytes. Consequently, we found p75 knockdown improved the pathologic morphology with reduced neuron damage, water content, permeability of blood-brain barrier and inflammation in ICH rat brain tissues. Besides, Knockdown of p75 decreased neuronal apoptosis and inactivated mTOR signaling pathway, but it elevated the levels of autophagy-related proteins. results were reproduced in experiments. This study demonstrated that knockdown of p75 could promote neuronal autophagy and reduce neuronal apoptosis via inactivating the mTOR pathway. We hope these findings could provide new therapeutic options for ICH treatment.