Guard Cells Integrate Light and Temperature Signals to Control Stomatal Aperture

High temperature promotes guard cell expansion, which opens stomatal pores to facilitate leaf cooling. How the high-temperature signal is perceived and transmitted to regulate stomatal aperture is, however, unknown. Here, we used a reverse-genetics approach to understand high temperature-mediated st...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2020-03, Vol.182 (3), p.1404-1419
Main Authors: Kostaki, Kalliopi-Ioanna, Coupel-Ledru, Aude, Bonnell, Verity C, Gustavsson, Mathilda, Sun, Peng, McLaughlin, Fiona J, Fraser, Donald P, McLachlan, Deirdre H, Hetherington, Alistair M, Dodd, Antony N, Franklin, Keara A
Format: Article
Language:English
Subjects:
14-3-3 Proteins - genetics
14-3-3 Proteins - metabolism
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Life Sciences
Plant Stomata - genetics
Plant Stomata - metabolism
Proton-Translocating ATPases - genetics
Proton-Translocating ATPases - metabolism
Signal Transduction - genetics
Signal Transduction - physiology
Temperature
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Summary:High temperature promotes guard cell expansion, which opens stomatal pores to facilitate leaf cooling. How the high-temperature signal is perceived and transmitted to regulate stomatal aperture is, however, unknown. Here, we used a reverse-genetics approach to understand high temperature-mediated stomatal opening in Arabidopsis ( ). Our findings reveal that high temperature-induced guard cell movement requires components involved in blue light-mediated stomatal opening, suggesting cross talk between light and temperature signaling pathways. The molecular players involved include phototropin photoreceptors, plasma membrane H -ATPases, and multiple members of the 14-3-3 protein family. We further show that phototropin-deficient mutants display impaired rosette evapotranspiration and leaf cooling at high temperatures. Blocking the interaction of 14-3-3 proteins with their client proteins severely impairs high temperature-induced stomatal opening but has no effect on the induction of heat-sensitive guard cell transcripts, supporting the existence of an additional intracellular high-temperature response pathway in plants.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.19.01528