Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens

• Published in: Tropical animal health and production 2010-12, Vol.42 (8), p.1855-1860
• Main Authors: V. J, Rejish Kumar, Seo, Byeong Joo, Mun, Mi Ran, Kim, Chul-Joong, Lee, Insun, Kim, Hongik, Park, Yong-Ha
• Format: Article
• Language: English
• Subjects: Animals; Antibiotic resistance; Antibiotic sensitivity; Antibiotics; Antimicrobial activity; Bacteria; Base Sequence; Bile; Biomedical and Life Sciences; Coronavirus; COVID-19; Drug Resistance, Multiple, Bacterial - genetics; Enteric pathogens; Gastric juice; Gastric Juice - microbiology; Gastroenteritis; Gastroenteritis, Transmissible, of Swine - drug therapy; Gastroenteritis, Transmissible, of Swine - microbiology; Gastrointestinal tract; Gastrointestinal Tract - microbiology; Lactobacillus; Lactobacillus plantarum; Lactobacillus plantarum - genetics; Lactobacillus salivarius; Life Sciences; Molecular Sequence Data; Original Research; Pathogens; probiotics; Probiotics - pharmacology; Probiotics - therapeutic use; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA - veterinary; Survival; Sus scrofa - microbiology; Swine; Transmissible gastroenteritis; Transmissible gastroenteritis coronavirus; Transmissible gastroenteritis virus - drug effects; Veterinary Medicine/Veterinary Science; Zoology
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-010-9648-5

A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates for animal application after proper in vivo experiments.