Effects of mechanical ventilation with different tidal volume on oxidative stress and antioxidant in lung

Purpose The aim of this study was to investigate the changes in oxidative stress and antioxidants in lung tissue under different tidal volume ventilation conditions. Methods Forty-eight male Wistar rats were randomized into four groups, namely, group C, the control group, which was not ventilated, a...

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Bibliographic Details
Published in:Journal of anesthesia 2015-06, Vol.29 (3), p.346-351
Main Authors: Sun, Zhen-Tao, Yang, Chun-Yao, Miao, Li-Jun, Zhang, Shan-Feng, Han, Xue-Ping, Ren, Su-En, Sun, Xue-Qing, Cao, Ya-Nan
Format: Article
Language:English
Subjects:
Analysis
Anesthesiology
Animals
Antioxidants
Antioxidants - metabolism
Critical Care Medicine
Emergency Medicine
Enzymes
Intensive
Lung - metabolism
Male
Malondialdehyde - metabolism
Medicine
Medicine & Public Health
Original
Original Article
Oxidative stress
Oxidative Stress - physiology
Pain Medicine
Rats
Rats, Wistar
Respiration, Artificial - methods
Tidal Volume - physiology
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Summary:Purpose The aim of this study was to investigate the changes in oxidative stress and antioxidants in lung tissue under different tidal volume ventilation conditions. Methods Forty-eight male Wistar rats were randomized into four groups, namely, group C, the control group, which was not ventilated, and groups C1, C2 and C3, the treatment groups, which were ventilated for 2 h with tidal volumes of 8, 30 and 42 ml/kg, respectively. The right middle lobe was assayed for malondialdehyde (MDA), the right posterior lobe was assayed using Western blotting for Nrf2, GCLm and SrX1 and the left lobe was assayed for Nrf2, GCLm and SrX1 mRNA. Results The MDA levels were increased in the three treatment groups, with MDA levels highest in group C3 and lowest in group C1 (C3 > C2 > C1) (all P   C2 > C1) (all P   0.05). A Western blot analysis showed that Nrf2, GCLm and SrX1 expression was highest in group C3 and lowest in group C1 (C3 > C2 > C1) (all P   0.05). Conclusions Oxidative stress and antioxidant enzyme levels in the lungs of rats were positively associated with the tidal volumes of mechanical ventilation, suggesting that higher tidal volumes cause more severe oxidative stress and increased antioxidant responses.
ISSN:0913-8668
1438-8359
DOI:10.1007/s00540-014-1954-z