Selection of staphylococcal enterotoxin B (SEB)-binding peptide using phage display technology

• Published in: Biochemical and biophysical research communications 2008-05, Vol.370 (1), p.104-108
• Main Authors: Soykut, Esra Acar, Dudak, Fahriye Ceyda, Boyacı, İsmail Hakkı
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; ANTIBODIES; Bacteriophage M13 - genetics; Bacteriophage M13 - isolation & purification; BACTERIOPHAGES; CALORIMETRY; ELISA; Enterotoxins - chemistry; ENZYME IMMUNOASSAY; Enzyme-Linked Immunosorbent Assay; FOOD; Isothermal titration calorimetry (ITC); Molecular Sequence Data; Peptide; Peptide Library; PEPTIDES; Peptides - chemistry; Peptides - genetics; Peptides - isolation & purification; Phage display; Sequence Analysis, Protein; STRUCTURAL CHEMICAL ANALYSIS; Surface Plasmon Resonance; Surface plasmon resonance (SPR); TITRATION
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.03.065

In this study, peptides were selected to recognize staphylococcal enterotoxin B (SEB) which cause food intoxication and can be used as a biological war agent. By using commercial M13 phage library, single plaque isolation of 38 phages was done and binding affinities were investigated with phage-ELISA. The specificities of the selected phage clones showing high affinity to SEB were checked by using different protein molecules which can be found in food samples. Furthermore, the affinities of three selected phage clones were determined by using surface plasmon resonance (SPR) sensors. Sequence analysis was realized for three peptides showing high binding affinity to SEB and WWRPLTPESPPA, MNLHDYHRLFWY, and QHPQINQTLYRM amino acid sequences were obtained. The peptide sequence with highest affinity to SEB was synthesized with solid phase peptide synthesis technique and thermodynamic constants of the peptide–SEB interaction were determined by using isothermal titration calorimetry (ITC) and compared with those of antibody–SEB interaction. The binding constant of the peptide was determined as 4.2 ± 0.7 × 10 5 M −1 which indicates a strong binding close to that of antibody.