Adipose stem cells from patients with Crohn's disease show a distinctive DNA methylation pattern

Crohn's disease (CD) is characterized by persistent inflammation and ulceration of the small or large bowel, and expansion of mesenteric adipose tissue, termed creeping fat (CF). We previously demonstrated that human adipose-derived stem cells (hASCs) from CF of patients with CD exhibit dysfunc...

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Bibliographic Details
Published in:Clinical epigenetics 2020-04, Vol.12 (1), p.53-53, Article 53
Main Authors: Serena, Carolina, Millan, Monica, Ejarque, Miriam, Saera-Vila, Alfonso, Maymó-Masip, Elsa, Núñez-Roa, Catalina, Monfort-Ferré, Diandra, Terrón-Puig, Margarida, Bautista, Michelle, Menacho, Margarita, Martí, Marc, Espin, Eloy, Vendrell, Joan, Fernández-Veledo, Sonia
Format: Article
Language:English
Subjects:
Adipose tissue
Analysis
Biotechnology industries
Cell differentiation
Criminal investigation
Crohn's disease
Deoxyribonucleic acid
Diseases
DNA
DNA binding proteins
DNA methylation
Epigenetic inheritance
Epigenetics
Gastrointestinal diseases
Gene expression
Genes
Genetic research
Genomes
Homeostasis
Immune response
Immune system
Inflammation
Intestine
Leukocytes (mononuclear)
Medical research
Metabolism
Methylation
Necrosis
Peripheral blood mononuclear cells
Phagocytes
Phenotypes
Remission
Stem cells
Tumor necrosis factor-α
Tumors
Zinc finger proteins
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Summary:Crohn's disease (CD) is characterized by persistent inflammation and ulceration of the small or large bowel, and expansion of mesenteric adipose tissue, termed creeping fat (CF). We previously demonstrated that human adipose-derived stem cells (hASCs) from CF of patients with CD exhibit dysfunctional phenotypes, including a pro-inflammatory profile, high phagocytic capacity, and weak immunosuppressive properties. Importantly, these phenotypes persist in patients in remission and are found in all adipose depots explored including subcutaneous fat. We hypothesized that changes in hASCs are a consequence of epigenetic modifications. We applied epigenome-wide profiling with a methylation array (Illumina EPIC/850k array) and gene expression analysis to explore the impact of CD on the methylation signature of hASCs isolated from the subcutaneous fat of patients with CD and healthy controls (n = 7 and 5, respectively; cohort I). Differentially methylated positions (p value cutoff
ISSN:1868-7075
1868-7083
1868-7083
1868-7075
DOI:10.1186/s13148-020-00843-3