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Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards
In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer conta...
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Published in: | European journal of clinical microbiology & infectious diseases 2020-06, Vol.39 (6), p.1059-1061 |
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container_title | European journal of clinical microbiology & infectious diseases |
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creator | La Scola, Bernard Le Bideau, Marion Andreani, Julien Hoang, Van Thuan Grimaldier, Clio Colson, Philippe Gautret, Philippe Raoult, Didier |
description | In a preliminary clinical study, we observed that the combination of hydroxychloroquine and azithromycin was effective against SARS-CoV-2 by shortening the duration of viral load in Covid-19 patients. It is of paramount importance to define when a treated patient can be considered as no longer contagious. Correlation between successful isolation of virus in cell culture and Ct value of quantitative RT-PCR targeting E gene suggests that patients with Ct above 33–34 using our RT-PCR system are not contagious and thus can be discharged from hospital care or strict confinement for non-hospitalized patients. |
doi_str_mv | 10.1007/s10096-020-03913-9 |
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subjects | Animals Azithromycin Bacteriology Betacoronavirus - isolation & purification Betacoronavirus - physiology Biomedical and Life Sciences Biomedicine Biotechnology Brief Report Cardiology and cardiovascular system Cell culture Chlorocebus aethiops Coronavirus Infections - diagnosis Coronavirus Infections - virology COVID-19 E protein Emerging diseases France Human health and pathology Humans Hydroxychloroquine Infectious diseases Internal Medicine Life Sciences Medical Microbiology Microbiology and Parasitology Pandemics Parasitology Patient Discharge Patients Pneumonia, Viral - diagnosis Pneumonia, Viral - virology Polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA RNA, Viral - analysis SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Specimen Handling - standards Vero Cells Viral diseases Viral Load Virology Viruses |
title | Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards |
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