Mass Spectrometry Based-Proteomic Analysis of Anisakis spp.: A Preliminary Study towards a New Diagnostic Tool

Anisakiasis is nowadays a well-known infection, mainly caused by the accidental ingestion of larvae, following the consumption of raw or undercooked fishes and cephalopods. Due to the similarity of symptoms with those of common gastrointestinal disorders, this infection is often underestimated, and...

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Bibliographic Details
Published in:Genes 2020-06, Vol.11 (6), p.693
Main Authors: Marzano, Valeria, Pane, Stefania, Foglietta, Gianluca, Levi Mortera, Stefano, Vernocchi, Pamela, Onetti Muda, Andrea, Putignani, Lorenza
Format: Article
Language:English
Subjects:
Animals
Anisakiasis - diagnosis
Anisakiasis - genetics
Anisakiasis - parasitology
Anisakis
Anisakis - genetics
Anisakis - pathogenicity
Biotyping
Chromatography
Endoscopy
Fishes - genetics
Fishes - parasitology
Fungi
Gastrointestinal diseases
Identification
Ions
Laboratories
Larva - pathogenicity
Lasers
Mass spectrometry
Mass spectroscopy
Nematodes
Parasites
Particle size
Pore size
Proteins
Proteome - genetics
Proteomes
Proteomics
Scientific imaging
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Anisakiasis is nowadays a well-known infection, mainly caused by the accidental ingestion of larvae, following the consumption of raw or undercooked fishes and cephalopods. Due to the similarity of symptoms with those of common gastrointestinal disorders, this infection is often underestimated, and the need for new specific diagnostic tools is becoming crucial. Given the remarkable impact that MALDI-TOF MS biotyping had in the last decade in clinical routine practice for the recognition of bacterial and fungi strains, a similar scenario could be foreseen for the identification of parasites, such as nematodes. In this work, a MALDI-TOF MS profiling of proteome was pursued with a view to constructing a first spectral library for the diagnosis of infections. At the same time, a shotgun proteomics approach by LC-ESI-MS/MS was performed on the two main fractions obtained from protein extraction, to evaluate the protein species enriched by the protocol. A set of MALDI-TOF MS signals associated with proteins originating in the ribosomal fraction of the nematode extract was selected as a potential diagnostic tool for the identification of spp.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes11060693