In Vitro and Randomized, Double‐Blind, Placebo‐Controlled Trial to Determine the Efficacy and Safety of Nine Antiacne Medicinal Plants

The present in vitro and randomized, double‐blind, placebo‐controlled trial aims to determine the efficacy and safety of nine Mediterranean antiacne medicinal plants. The antimicrobial, antisebum, and anti-inflammatory activities of the plant extracts were evaluated in cells from the immortalized hu...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-14
Main Authors: Saad, Bashar, Amin, Riyad, AboFarekh, Basheer, Fulder, Stephen, Kmail, Abdalsalam, Khamaysi, Iyad, Said, Omar, Daraghmeh, Jamila
Format: Article
Language:English
Subjects:
Acne
Anti-inflammatory agents
Cell culture
Chloramphenicol
Clinical trials
Cytokines
Cytotoxicity
Dehydrogenases
Double-blind studies
Herbal medicine
Immune system
Inflammation
Interleukin 6
Keratinocytes
Laboratories
Lipopolysaccharides
Medical research
Medicinal plants
Medicine, Botanic
Medicine, Herbal
Mitogens
Monocytes
Nitric oxide
Penicillin
Physiology
Phytochemicals
Plant extracts
Seeds
Tumor necrosis factor-α
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Summary:The present in vitro and randomized, double‐blind, placebo‐controlled trial aims to determine the efficacy and safety of nine Mediterranean antiacne medicinal plants. The antimicrobial, antisebum, and anti-inflammatory activities of the plant extracts were evaluated in cells from the immortalized human keratinocytes (HaCaT) and human monocytic cell line (THP-1) as well as in a double-blind, randomized, and placebo‐controlled trial. Most of the extracts showed no significant cytotoxic effects on HaCaT cells up to 250 μg/ml. Inula helenium (IH) and Saponaria officinalis (SO) inhibited sebum production at 90 μg/ml and 30 μg/ml, respectively. The inhibition effect of SO on the growth of Cutibacterium acnes was 1.2 times higher than that of chloramphenicol. IH and SO extracts significantly inhibited the lipopolysaccharide- (LPS-) induced IL-6 and TNF-α production in THP-1 cells reaching the control levels of untreated cells at a concentration of 250 μg/ml. SO, IH, and Solanum nigrum (SN) extracts inhibited the nitric oxide (NO) production in a dose-dependent manner. Based on these results, an antiacne herbal cream (AHC) was prepared from different portions of extracts from SO, IH, and SN, and its efficacy was evaluated in a double-blind, randomized, and controlled efficacy study with 41 acne patients, ages 18–24, who were asked to apply AHC (n = 27) or a placebo (n = 14) two to three times daily for six weeks. Results obtained indicate that the AHC has unique synergistic effects that halt sebum production, combined with highly antiseptic and anti-inflammatory activity, in which 54.95% (t = 19.37 P
ISSN:1741-427X
1741-4288
DOI:10.1155/2020/3231413