UPLC‐HRMS‐based untargeted metabolic profiling reveals changes in chickpea (Cicer arietinum) metabolome following long‐term drought stress

• Published in: Plant, cell and environment cell and environment, 2019-01, Vol.42 (1), p.115-132
• Main Authors: Khan, Naeem, Bano, Asghari, Rahman, Mohammad Atikur, Rathinasabapathi, Bala, Babar, Md Ali
• Format: Article
• Language: English
• Subjects: Accumulation; Alanine; Allantoin; Amino acids; Arginine; Aspartic acid; Biosynthesis; Chickpeas; Chlorophyll; Chlorophyll - metabolism; Choline; Chromatography, High Pressure Liquid; Cicer - metabolism; Cicer - physiology; Dehydration; Discriminant analysis; Drought; Drought resistance; Environmental impact; Genetic diversity; Glucosamine; Guanine; Histidine; Identification methods; Isoleucine; Leaves; Liquid chromatography; Mass Spectrometry; Mass spectroscopy; metabolic pathway; Metabolism; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; Moisture content; Original; Phenylalanine; Physiology; Plant Leaves - metabolism; Plant Roots - metabolism; Plant Shoots - metabolism; Proline; Stress; tRNA Asp; Tryptophan; Tyrosine; UPLC‐HRMS analysis; Water - metabolism; Water content; Water regimes; water stress; Weight
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.13195

Genetic improvement for drought tolerance in chickpea requires a solid understanding of biochemical processes involved with different physiological mechanisms. The objective of this study is to demonstrate genetic variations in altered metabolic levels in chickpea varieties (tolerant and sensitive) grown under contrasting water regimes through ultrahigh‐performance liquid chromatography/high‐resolution mass spectrometry‐based untargeted metabolomic profiling. Chickpea plants were exposed to drought stress at the 3‐leaf stage for 25 days, and the leaves were harvested at 14 and 25 days after the imposition of drought stress. Stress produced significant reduction in chlorophyll content, Fv/Fm, relative water content, and shoot and root dry weight. Twenty known metabolites were identified as most important by 2 different methods including significant analysis of metabolites and partial least squares discriminant analysis. The most pronounced increase in accumulation due to drought stress was demonstrated for allantoin, l‐proline, l‐arginine, l‐histidine, l‐isoleucine, and tryptophan. Metabolites that showed a decreased level of accumulation under drought conditions were choline, phenylalanine, gamma‐aminobutyric acid, alanine, phenylalanine, tyrosine, glucosamine, guanine, and aspartic acid. Aminoacyl‐tRNA and plant secondary metabolite biosynthesis and amino acid metabolism or synthesis pathways were involved in producing genetic variation under drought conditions. Metabolic changes in light of drought conditions highlighted pools of metabolites that affect the metabolic and physiological adjustment in chickpea that reduced drought impacts. Drought stress is one of the major problems in chickpea‐growing areas. Though drought stress changes biochemical mechanisms in plants, however, little is known about the complex metabolic regulation for genetic improvement in chickpea under drought stress environments. This study was conducted to identify changes at different metabolites in two chickpea varieties contrasting for drought tolerance under drought and control conditions. This study also demonstrates the metabolic pathways potentially involved in drought tolerance mechanisms in chickpea.