Genomic and Transcriptomic Evidence Supports Methane Metabolism in Archaeoglobi

Euryarchaeal lineages have been believed to have a methanogenic last common ancestor. However, members of euryarchaeal have long been considered nonmethanogenic and their evolutionary history remains elusive. Here, three high-quality metagenomic-assembled genomes (MAGs) retrieved from high-temperatu...

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Bibliographic Details
Published in:mSystems 2020-03, Vol.5 (2)
Main Authors: Liu, Yi-Fan, Chen, Jing, Zaramela, Livia S, Wang, Li-Ying, Mbadinga, Serge Maurice, Hou, Zhao-Wei, Wu, Xiao-Lin, Gu, Ji-Dong, Zengler, Karsten, Mu, Bo-Zhong
Format: Article
Language:English
Subjects:
Archaeoglobi
BASIC BIOLOGICAL SCIENCES
Biosynthesis
Coenzyme M
Dehydrogenases
Divergence
Ecological and Evolutionary Science
Enzymes
Evolution
Fermentation
Genes
Genomes
HGT
horizontal gene transfer
Hot springs
Hydrocarbons
MCR complex
Metagenomics
metatranscriptomics
Methanogenesis
methanogens
methyl-coenzyme M reductase complex
Methyltransferase
Microbiology
National parks
oil reservoir
Phylogeny
Proteins
Reductase
Sulfate reduction
Trees
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Summary:Euryarchaeal lineages have been believed to have a methanogenic last common ancestor. However, members of euryarchaeal have long been considered nonmethanogenic and their evolutionary history remains elusive. Here, three high-quality metagenomic-assembled genomes (MAGs) retrieved from high-temperature oil reservoir and hot springs, together with three newly assembled MAGs from previously reported hot spring metagenomes, are demonstrated to represent a novel genus of , " Methanomixophus." All " Methanomixophus" MAGs encode an M methyltransferase (MTR) complex and a traditional type of methyl-coenzyme M reductase (MCR) complex, which is different from the divergent MCR complexes found in " Polytropus marinifundus." In addition, " Methanomixophus dualitatem" MAGs preserve the genomic capacity for dissimilatory sulfate reduction. Comparative phylogenetic analysis supports a laterally transferred origin for an MCR complex and vertical heritage of the MTR complex in this lineage. Metatranscriptomic analysis revealed concomitant activity of hydrogen-dependent methylotrophic methanogenesis and heterotrophic fermentation within populations of " Methanomixophus hydrogenotrophicum" in a high-temperature oil reservoir. Current understanding of the diversity, biology, and ecology of is very limited, especially considering how few of the known phyla have been cultured or genomically explored. The reconstruction of " Methanomixophus" MAGs not only expands the known range of metabolic versatility of the members of but also suggests that the phylogenetic distribution of MCR and MTR complexes is even wider than previously anticipated.
ISSN:2379-5077
2379-5077
DOI:10.1128/mSystems.00651-19