Sapovirus detection and quantification in fecal samples from allogeneic hematopoietic stem cell transplant recipients

• Published in: Virusdisease 2020-09, Vol.31 (3), p.374-377
• Main Authors: Neres Silva, Thairiny, Dábilla, Nathânia, Santos Corrêa, Thaís, de Moraes Arantes, Adriano, Souza, Menira
• Format: Article
• Language: English
• Subjects: Allografts; Biochemistry; Biomedical and Life Sciences; Bone marrow; Cell Biology; Conflicts of interest; Diarrhea; Feces; Gastroenteritis; Genotype & phenotype; Genotypes; Graft versus host disease; Hematopoietic stem cells; Immunocompromised hosts; Infections; Life Sciences; Longitudinal studies; Microbiology; Patients; Protein Structure; Sapovirus; Short Communication; Stem cell transplantation
• ISSN: 2347-3584; 2347-3517
• DOI: 10.1007/s13337-020-00583-1

Sapovirus are important agents of acute gastroenteritis (AGE) and they are associated with outbreaks and sporadic cases worldwide. They infect people of all ages, but mainly children, the elderly and immunocompromised individuals are affected. The aim of this study was investigate sapovirus and to determine viral loads in fecal samples from patient undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Fecal samples were submitted to extraction of the genetic material using a commercial kit, and RT-qPCR TaqMan was used for sapovirus screening and determination of viral loads, using a standard curve with serial dilutions of a recombinant plasmid. Positive samples were sequence by Sanger method. Sapovirus was detected in one patient, 5.3% (1/19). Viral excretion lasted for 16 days. Viral load varied from 1.73 × 10 6 to 8.97 × 10 6  GC/g. One of the positive samples was characterized as GI.1 genotype. This is the first study to determine sapovirus loads in samples from allo-HSCT and to identify GI.1 genotype in immunocompromised patients.