Sharing a β-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium

• Published in: Applied and environmental microbiology 2020-10, Vol.86 (20)
• Main Authors: Centanni, Manuela, Sims, Ian M, Bell, Tracey J, Biswas, Ambarish, Tannock, Gerald W
• Format: Article
• Language: English
• Subjects: Bacteroides; Bacteroides - metabolism; Barley; Beta cells; beta-Glucans - metabolism; Carbohydrates; Cell culture; Clostridiaceae - metabolism; Clostridiales - metabolism; Consortia; Continuous culture; Depolymerization; Deprivation; Dietary fiber; Eavesdropping; Food Microbiology; Gene expression; Glucan; Glucose; Hordeum - chemistry; Intestinal microflora; Loci; Microbial Consortia; Microbiota; Oligosaccharides; Stringent response; Substrates; Transcription; Transcriptome; Vitamin B12; β-Glucan
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01651-20

Whole-transcriptome analysis was used to investigate the molecular interplay between three bacterial species that are members of the human gut microbiota. , , and formed associations in cocultures fed barley β-glucan, a constituent of dietary fiber. depolymerized β-glucan and released, but did not utilize, 3- -β-cellobiosyl-d-glucose (DP3) and 3- -β-cellotriosyl-d-glucose (DP4). These oligosaccharides provided growth substrates for and with a preference for DP4 in the case of the latter species. There was increased transcription of a mixed-linkage-β-glucan utilization locus, as well as carbohydrate transporters in and when in batch coculture. Increased transcription of the β-glucan utilization locus did not occur in continuous culture. Evidence for interactions relating to provision of cobalamin, alterations to signaling, and modulation of the "stringent response" (an adaptation to nutrient deprivation) were detected. Overall, we established a bacterial consortium based on barley β-glucan , which can be used to investigate aspects of the functional blueprint of the human gut microbiota. The microbial community, mostly composed of bacterial species, residing in the human gut degrades and ferments polysaccharides derived from plants (dietary fiber) that would not otherwise be digested. In this way, the collective metabolic actions of community members extract additional energy from the human diet. While the variety of bacteria present in the microbial community is well known, the formation of bacterial consortia, and the consequent interactions that result in the digestion of dietary polysaccharides, has not been studied extensively. The importance of our work was the establishment, under laboratory conditions, of a consortium of gut bacteria that formed around a dietary constituent commonly present in cereals. This enabled the metabolic interplay between the bacterial species to be studied. This kind of knowledge is required to construct an interactive, metabolic blueprint of the microbial community that inhabits the human gut.