Dietary Supplementation with Spray-Dried Porcine Plasma Attenuates Colon Inflammation in a Genetic Mouse Model of Inflammatory Bowel Disease

• Published in: International journal of molecular sciences 2020-09, Vol.21 (18), p.6760
• Main Authors: Miró, Lluïsa, Amat, Concepció, Rosell-Cardona, Cristina, Campbell, Joy M, Polo, Javier, Pérez-Bosque, Anna, Moretó, Miquel
• Format: Article
• Language: English
• Subjects: Animals; Blood Proteins - pharmacology; Colitis - drug therapy; Colitis - metabolism; Colon - drug effects; Colon - metabolism; Cytokines - metabolism; Diet; Dietary Supplements; Disease Models, Animal; Immunity, Mucosal - drug effects; Inflammation - drug therapy; Inflammatory Bowel Diseases - drug therapy; Inflammatory Bowel Diseases - metabolism; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Mice; Mice, Knockout; Plasma - metabolism; Swine - metabolism
• ISSN: 1422-0067; 1422-0067
• DOI: 10.3390/ijms21186760

Dietary supplementation with spray-dried porcine plasma (SDP) can modulate the immune response of gut-associated lymphoid tissue. SDP supplementation reduces acute mucosal inflammation, as well as chronic inflammation associated with aging. The aim of this study was to analyze if SDP supplementation could ameliorate colitis in a genetic mouse model of inflammatory bowel disease (IBD). Wild-type mice and knockout (KO) mice were administered a control diet or an SDP-supplemented diet from day 21 (weaning) until day 56. The histopathological index, epithelial barrier, and intestinal immune system were analyzed in the colonic mucosa. KO mice had higher epithelial permeability, increased and expression, and lower abundance of E-cadherin and (all < 0.001). SDP prevented these effects (all < 0.05) and decreased the colonic inflammation observed in KO mice, reducing neutrophil and monocyte infiltration and activation and the percentage of activated T helper lymphocytes in the colonic mucosa (all < 0.05). SDP also diminished proinflammatory cytokine expression and increased the anti-inflammatory IL-10 concentration in the colonic mucosa (all < 0.05). In conclusion, dietary supplementation with SDP enhances colon barrier function and reduces mucosal inflammation in a mouse model of IBD.