High abundance of the colistin resistance gene mcr-1 in chicken gut-bacteria in Bangladesh

Colistin is considered a last-resort reserved drug for the treatment of critical human infections by Gram-negative bacteria. Phenotypic colistin-resistance is strongly associated with plasmid-mediated mobile colistin resistance ( mcr ) genes. The mcr -bearing Enterobacteriaceae have been detected in...

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Published in:Scientific reports 2020-10, Vol.10 (1), p.17292, Article 17292
Main Authors: Islam, Salequl, Urmi, Umme Laila, Rana, Masud, Sultana, Fahmida, Jahan, Nusrat, Hossain, Billal, Iqbal, Samiul, Hossain, Md. Moyazzem, Mosaddek, Abu Syed Md, Nahar, Shamsun
Format: Article
Language:English
Subjects:
631/326/22/1434
692/700/478/174
Animals
Anti-Bacterial Agents - pharmacology
Bangladesh
Chickens - microbiology
Colistin - pharmacology
Dose-Response Relationship, Drug
Drug Resistance, Bacterial - genetics
Escherichia coli Proteins
Feces - microbiology
Gastrointestinal Microbiome - drug effects
Gastrointestinal Microbiome - genetics
Humanities and Social Sciences
multidisciplinary
Phenotype
Science
Science (multidisciplinary)
Transferases (Other Substituted Phosphate Groups)
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Summary:Colistin is considered a last-resort reserved drug for the treatment of critical human infections by Gram-negative bacteria. Phenotypic colistin-resistance is strongly associated with plasmid-mediated mobile colistin resistance ( mcr ) genes. The mcr -bearing Enterobacteriaceae have been detected in many countries from environments, animals, and humans. This study investigated phenotypic colistin-resistance and the distribution of mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5 genes in chicken-gut bacteria in Bangladesh. Bacteria were isolated from poultry- and native-chicken droppings, and their susceptibilities to colistin were determined by agar dilution and E-test minimal inhibitory concentration (MIC) measurements. Multiplex polymerase chain reactions detected mcr-1 to mcr-5 genes. Overall, 61.7% (92/149) of the isolates showed colistin resistance by agar dilution assessment (MIC > 2.0 μg/mL). The phenotypic resistance was observed considerably higher in poultry-chicken isolates (64.6%, 64/99) than in native-chicken isolates (56%, 28/50; p  = 0.373). All the resistant isolates showed MIC levels between > 2 and > 128 μg/mL. The mcr -genes ( mcr-1 and mcr-2 combined ) were detected more in poultry gut bacteria (36.4%) than native-chicken isolates (20%, p  = 0.06). Despite bacteria sources, mcr- genes appeared to be significantly associated with phenotypic colistin-resistance phenomena ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-74402-4