Sarcomere integrated biosensor detects myofilament-activating ligands in real time during twitch contractions in live cardiac muscle

• Published in: Journal of molecular and cellular cardiology 2020-10, Vol.147, p.49-61
• Main Authors: Vetter, Anthony D., Martin, Ashley A., Thompson, Brian R., Thomas, David D., Metzger, Joseph M.
• Format: Article
• Language: English
• Subjects: Myofilament; Myosin, contraction, calcium; Troponin
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1016/j.yjmcc.2020.07.012

The sarcomere is the functional unit of cardiac muscle, essential for normal heart function. To date, it has not been possible to study, in real time, thin filament-based activation dynamics in live cardiac muscle. We report here results from a cardiac troponin C (TnC) FRET-based biosensor integrated into the cardiac sarcomere via stoichiometric replacement of endogenous TnC. The TnC biosensor provides, for the first time, evidence of multiple thin filament activating ligands, including troponin I interfacing with TnC and cycling myosin, during a cardiac twitch. Results show that the TnC FRET biosensor transient significantly precedes that of peak twitch force. Using small molecules and genetic modifiers known to alter sarcomere activation, independently of the intracellular Ca2+ transient, the data show that the TnC biosensor detects significant effects of the troponin I switch domain as a sarcomere-activating ligand. Interestingly, the TnC biosensor also detected the effects of load-dependent altered myosin cycling, as shown by a significant delay in TnC biosensor transient inactivation during the isometric twitch. In addition, the TnC biosensor detected the effects of myosin as an activating ligand during the twitch by using a small molecule that directly alters cross-bridge cycling, independently of the intracellular Ca2+ transient. Collectively, these results aid in illuminating the basis of cardiac muscle contractile activation with implications for gene, protein, and small molecule-based strategies designed to target the sarcomere in regulating beat-to-beat heart performance in health and disease. [Display omitted] •Cardiac troponin C (TnC) FRET-based biosensor was integrated into the sarcomere•TnC biosensor detected thin filament activation in real time in live cardiac muscle during twitch contractions•The TnC biosensor detected multiple ligands of activation, including Ca2+, TnI, myosin and physiological loading