TRPV4 calcium influx controls sclerostin protein loss independent of purinergic calcium oscillations

• Published in: Bone (New York, N.Y.) N.Y.), 2020-07, Vol.136, p.115356-115356, Article 115356
• Main Authors: Williams, Katrina M., Leser, Jenna M., Gould, Nicole R., Joca, Humberto C., Lyons, James S., Khairallah, Ramzi J., Ward, Christopher W., Stains, Joseph P.
• Format: Article
• Language: English
• Subjects: Calcium oscillations; Calcium signaling; NOX2; Osteocyte; Sclerostin; TRPV4
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/j.bone.2020.115356

Skeletal remodeling is driven in part by the osteocyte's ability to respond to its mechanical environment by regulating the abundance of sclerostin, a negative regulator of bone mass. We have recently shown that the osteocyte responds to fluid shear stress via the microtubule network-dependent activation of NADPH oxidase 2 (NOX2)-generated reactive oxygen species and subsequent opening of TRPV4 cation channels, leading to calcium influx, activation of CaMKII, and rapid sclerostin protein downregulation. In addition to the initial calcium influx, purinergic receptor signaling and calcium oscillations occur in response to mechanical load and prior to rapid sclerostin protein loss. However, the independent contributions of TRPV4-mediated calcium influx and purinergic calcium oscillations to the rapid sclerostin protein downregulation remain unclear. Here, we showed that NOX2 and TRPV4-dependent calcium influx is required for calcium oscillations, and that TRPV4 activation is both necessary and sufficient for sclerostin degradation. In contrast, calcium oscillations are neither necessary nor sufficient to acutely decrease sclerostin protein abundance. However, blocking oscillations with apyrase prevented fluid shear stress induced changes in osterix (Sp7), osteoprotegerin (Tnfrsf11b), and sclerostin (Sost) gene expression. In total, these data provide key mechanistic insights into the way bone cells translate mechanical cues to target a key effector of bone formation, sclerostin. •NOX2 and TRPV4-dependent Ca2+ influx is required for subsequent Ca2+ oscillations.•TRPV4 activation is necessary and sufficient for phosphorylation of CaMKII and rapid loss of sclerostin protein.•Ca2+ oscillations are neither necessary nor sufficient to activate CaMKII or decrease sclerostin protein.•Ca2+ oscillations contribute to long term regulation of osteocyte gene expression.