Replicate Aptima Assay for Quantifying Residual Plasma Viremia in Individuals on Antiretroviral Therapy

Detection of residual plasma viremia in antiretroviral therapy (ART)-suppressed HIV-infected individuals is critical for characterizing the latent reservoir and evaluating the impact of cure interventions. Ultracentrifugation-based single-copy assays are sensitive but labor intensive. Fully automate...

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Bibliographic Details
Published in:Journal of clinical microbiology 2020-11, Vol.58 (12)
Main Authors: Bakkour, Sonia, Deng, Xutao, Bacchetti, Peter, Grebe, Eduard, Montalvo, Leilani, Worlock, Andrew, Stone, Mars, Deeks, Steven G, Richman, Douglas D, Busch, Michael P
Format: Article
Language:English
Subjects:
Virology
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Summary:Detection of residual plasma viremia in antiretroviral therapy (ART)-suppressed HIV-infected individuals is critical for characterizing the latent reservoir and evaluating the impact of cure interventions. Ultracentrifugation-based single-copy assays are sensitive but labor intensive. Fully automated replicate testing using a standard clinical viral load assay was evaluated as a high-throughput alternative for the quantification of low-level viremia. Four plasma samples from blood donors with acute HIV-1 infection and one viral culture supernatant were serially diluted into 25-ml samples to nominal viral loads ranging from 39 to
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01400-20