A bacteriocin-based antimicrobial formulation to effectively disrupt the cell viability of methicillin-resistant Staphylococcus aureus (MRSA) biofilms

Antibiotic-resistant and biofilm-associated infections brought about by methicillin-resistant Staphylococcus aureus (MRSA) strains is a pressing issue both inside as well as outside nosocomial environments worldwide. Here, we show that a combination of two bacteriocins with distinct structural and f...

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Bibliographic Details
Published in:NPJ biofilms and microbiomes 2020-12, Vol.6 (1), p.58-58, Article 58
Main Authors: Kranjec, Christian, Ovchinnikov, Kirill V., Grønseth, Torstein, Ebineshan, Kumar, Srikantam, Aparna, Diep, Dzung B.
Format: Article
Language:English
Subjects:
631/326
631/326/22
631/326/46
Antibacterial activity
Antibiotic resistance
Antibiotics
Antimicrobial agents
Bacteriocins
Biofilms
Biomedical and Life Sciences
Cell viability
Drug resistance
Hospitals
Life Sciences
Medical Microbiology
Methicillin
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Nosocomial environments
Penicillin
Scanning electron microscopy
Staphylococcus aureus
Staphylococcus infections
Strains (organisms)
Structure-function relationships
β-Lactam antibiotics
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Summary:Antibiotic-resistant and biofilm-associated infections brought about by methicillin-resistant Staphylococcus aureus (MRSA) strains is a pressing issue both inside as well as outside nosocomial environments worldwide. Here, we show that a combination of two bacteriocins with distinct structural and functional characteristics, garvicin KS, and micrococcin P1, showed a synergetic antibacterial activity against biofilms produced in vitro by S. aureus , including several MRSA strains. In addition, this bacteriocin-based antimicrobial combination showed the ability to restore the sensitivity of the highly resilient MRSA strain ATCC 33591 to the β-lactam antibiotic penicillin G. By using a combination of bacterial cell metabolic assays, confocal and scanning electron microscopy, we show that the combination between garvicin KS, micrococcin P1, and penicillin G potently inhibit cell viability within S. aureus biofilms by causing severe cell damage. Together these data indicate that bacteriocins can be valuable therapeutic tools in the fight against biofilm-associated MRSA infections.
ISSN:2055-5008
2055-5008
DOI:10.1038/s41522-020-00166-4