Muscle memory: myonuclear accretion, maintenance, morphology, and miRNA levels with training and detraining in adult mice

Background In the context of mass regulation, ‘muscle memory’ can be defined as long‐lasting cellular adaptations to hypertrophic exercise training that persist during detraining‐induced atrophy and may facilitate future adaptation. The cellular basis of muscle memory is not clearly defined but may...

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Bibliographic Details
Published in:Journal of cachexia, sarcopenia and muscle sarcopenia and muscle, 2020-12, Vol.11 (6), p.1705-1722
Main Authors: Murach, Kevin A., Mobley, C. Brooks, Zdunek, Christopher J., Frick, Kaitlyn K., Jones, Savannah R., McCarthy, John J., Peterson, Charlotte A., Dungan, Cory M.
Format: Article
Language:English
Subjects:
Adaptation
Animals
Antibodies
Atrophy
Central nuclei
DNA methylation
Drinking water
Epigenetics
Female
H2B‐GFP
Hypertrophy
Hypotheses
Laboratories
Mice
Mice, Inbred C57BL
MicroRNAs - genetics
miR‐1
Motor Activity
Muscle damage
Muscle, Skeletal
Muscular system
Musculoskeletal system
Original
Satellite Cells, Skeletal Muscle
Software
Tet‐On
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Summary:Background In the context of mass regulation, ‘muscle memory’ can be defined as long‐lasting cellular adaptations to hypertrophic exercise training that persist during detraining‐induced atrophy and may facilitate future adaptation. The cellular basis of muscle memory is not clearly defined but may be related to myonuclear number and/or epigenetic changes within muscle fibres. Methods Utilizing progressive weighted wheel running (PoWeR), a novel murine exercise training model, we explored myonuclear dynamics and skeletal muscle miRNA levels with training and detraining utilizing immunohistochemistry, single fibre myonuclear analysis, and quantitative analysis of miRNAs. We also used a genetically inducible mouse model of fluorescent myonuclear labelling to study myonuclear adaptations early during exercise. Results In the soleus, oxidative type 2a fibres were larger after 2 months of PoWeR (P = 0.02), but muscle fibre size and myonuclear number did not return to untrained levels after 6 months of detraining. Soleus type 1 fibres were not larger after PoWeR but had significantly more myonuclei, as well as central nuclei (P 
ISSN:2190-5991
2190-6009
DOI:10.1002/jcsm.12617