Tobacco smoking induces metabolic reprogramming of renal cell carcinoma

• Published in: The Journal of clinical investigation 2021-01, Vol.131 (1), p.1-16
• Main Authors: Reigle, James, Secic, Dina, Biesiada, Jacek, Wetzel, Collin, Shamsaei, Behrouz, Chu, Johnson, Zang, Yuanwei, Zhang, Xiang, Talbot, Nicholas J, Bischoff, Megan E, Zhang, Yongzhen, Thakar, Charuhas V, Gaitonde, Krishnanath, Sidana, Abhinav, Bui, Hai, Cunningham, John T, Zhang, Qing, Schmidt, Laura S, Linehan, W Marston, Medvedovic, Mario, Plas, David R, Figueroa, Julio A Landero, Meller, Jarek, Czyzyk-Krzeska, Maria F
• Format: Article
• Language: English
• Subjects: Arsenic; Biomedical research; Cadmium; Carcinoma, Renal cell; Care and treatment; Cell metabolism; Cigarettes; Clear cell-type renal cell carcinoma; Clinical Medicine; Copper; Cytochrome-c oxidase; Development and progression; Gene expression; Genomes; Glutamine; Health aspects; Heavy metals; Histidine; Homeostasis; Kidney cancer; Metabolic pathways; Metabolism; Metabolites; Metabolomics; Mutation; Oxidative phosphorylation; Phosphorylation; Precision medicine; Risk factors; Smoking; Tobacco; Tobacco smoking; Transcription factors; Tumors; White people
• ISSN: 0021-9738; 1558-8238
• DOI: 10.1172/JCI140522

BACKGROUNDClear cell renal cell carcinoma (ccRCC) is the most common histologically defined renal cancer. However, it is not a uniform disease and includes several genetic subtypes with different prognoses. ccRCC is also characterized by distinctive metabolic reprogramming. Tobacco smoking (TS) is an established risk factor for ccRCC, with unknown effects on tumor pathobiology.METHODSWe investigated the landscape of ccRCCs and paired normal kidney tissues using integrated transcriptomic, metabolomic, and metallomic approaches in a cohort of white males who were long-term current smokers (LTS) or were never smokers (NS).RESULTSAll 3 Omics domains consistently identified a distinct metabolic subtype of ccRCCs in LTS, characterized by activation of oxidative phosphorylation (OXPHOS) coupled with reprogramming of the malate-aspartate shuttle and metabolism of aspartate, glutamate, glutamine, and histidine. Cadmium, copper, and inorganic arsenic accumulated in LTS tumors, showing redistribution among intracellular pools, including relocation of copper into the cytochrome c oxidase complex. A gene expression signature based on the LTS metabolic subtype provided prognostic stratification of The Cancer Genome Atlas ccRCC tumors that was independent of genomic alterations.CONCLUSIONThe work identified the TS-related metabolic subtype of ccRCC with vulnerabilities that can be exploited for precision medicine approaches targeting metabolic pathways. The results provided rationale for the development of metabolic biomarkers with diagnostic and prognostic applications using evaluation of OXPHOS status. The metallomic analysis revealed the role of disrupted metal homeostasis in ccRCC, highlighting the importance of studying effects of metals from e-cigarettes and environmental exposures.FUNDINGDepartment of Defense, Veteran Administration, NIH, ACS, and University of Cincinnati Cancer Institute.