The Subchronic Toxic Effects of Mosla chinensis Maxim in Normal Rats

• Published in: BioMed research international 2020, Vol.2020 (2020), p.1-20
• Main Authors: Liu, Zhiyong, Hong, Tao, Shu, Kun, Gu, Zhouyang, Cai, Pingdong, Liu, Li, Huang, Shenghong, Li, Longxue, Lei, Dan, Li, Shouming
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Biomarkers - blood; Biomarkers - urine; Blood Glucose - metabolism; Body Weight - drug effects; Chinese medicine; Control; Discriminant analysis; Distilled water; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - toxicity; Energy Metabolism - drug effects; Erythrocytes; Experiments; Female; Flora; Food; Gas flow; Gastroenteritis; Gastrointestinal Microbiome - drug effects; Gastrointestinal Motility - drug effects; Health aspects; Hematologic Tests; Herbal medicine; Identification and classification; Immunity - drug effects; Intestinal microflora; Intestine; Kidney - drug effects; Kidney - physiopathology; Laboratory animals; Lactic acid; Lactic acid bacteria; Lamiaceae - chemistry; Lipids - blood; Liver; Liver - drug effects; Liver - physiopathology; Medical research; Medicine, Botanic; Medicine, Herbal; Metabolism; Metabolites; Microbiota (Symbiotic organisms); Mosla; Organ Specificity - drug effects; Organs; Oxidation-Reduction - drug effects; Physiological effects; Principal Component Analysis; Principal components analysis; Rats, Sprague-Dawley; Statistical analysis; Toxicity; Toxicity testing; Toxicity Tests, Chronic; Tryptophan; Tumor necrosis factor-TNF; Uric acid; Uric acid metabolism; Variance analysis; Water pollution effects
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2020/4521586

Background. The aim of this work was to study the toxic effects and target organs of Mosla chinensis Maxim (MCM) in rats and provide theoretical basis for clinical medication. Methods. The subchronic toxicity study was conducted on 60 male and female SD rats using the fixed-dose method for the treatment groups and 20 male and female SD rats for the control. At the subchronic toxicity study, the water extract of MCM with fixed doses of 0.2 g/kg/day, 2 g/kg/day, and 20 g/kg/day was administered for 90 days intragastric, and the control group was given the same amount of distilled water. After 90 days, the general conditions of the rats were observed. Assessment on safety of the extract was conducted by a subchronic toxicity test which mainly examined alteration occurrence in gut flora and urine metabolism. Results. There was no significant difference in physical signs, reactivity, and stool characteristics in the four groups. Compared with the control group, the number of red blood cells in the male 2 g/kg/day group and the female 0.2 g/kg/day group was significantly different (P