Metabolic changes in mouse sperm during capacitation

Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm mod...

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Bibliographic Details
Published in:Biology of reproduction 2020-10, Vol.103 (4), p.791-801
Main Authors: Balbach, Melanie, Gervasi, Maria Gracia, Hidalgo, David Martin, Visconti, Pablo E, Levin, Lonny R, Buck, Jochen
Format: Article
Language:English
Subjects:
capacitation
energy production
glucose uptake
glycolysis
Metabolism
Observations
oxidative phosphorylation
Phosphorylation
Physiological aspects
Rodents
Sperm
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Summary:Mammalian sperm are stored in the epididymis in a dormant state. Upon ejaculation, they must immediately start producing sufficient energy to maintain motility and support capacitation. While this increased energy demand during capacitation is well established, it remains unclear how mouse sperm modify their metabolism to meet this need. We now show that capacitating mouse sperm enhance glucose uptake, identifying glucose uptake as a functional marker of capacitation. Using an extracellular flux analyzer, we show that glycolysis and oxidative phosphorylation increase during capacitation. Furthermore, this increase in oxidative phosphorylation is dependent on glycolysis, providing experimental evidence for a link between glycolysis and oxidative phosphorylation in mouse sperm. Summary Sentence During capacitation, mouse sperm increase the rates of both glycolysis and oxidative phosphorylation.
ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioaa114