Immune synapse instructs epigenomic and transcriptomic functional reprogramming in dendritic cells

Understanding the fate of dendritic cells (DCs) after productive immune synapses (postsynaptic DCs) with T cells during antigen presentation has been largely neglected in favor of deciphering the nuances of T cell activation and memory generation. Here, we describe that postsynaptic DCs switch their...

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Bibliographic Details
Published in:Science advances 2021-02, Vol.7 (6)
Main Authors: Alcaraz-Serna, Ana, Bustos-Morán, Eugenio, Fernández-Delgado, Irene, Calzada-Fraile, Diego, Torralba, Daniel, Marina-Zárate, Ester, Lorenzo-Vivas, Erika, Vázquez, Enrique, de Alburquerque, Juliana Barreto, Ruef, Nora, Gómez, Manuel José, Sánchez-Cabo, Fátima, Dopazo, Ana, Stein, Jens V, Ramiro, Almudena, Sánchez-Madrid, Francisco
Format: Article
Language:English
Subjects:
Cell Movement
Dendritic Cells
Epigenomics
Immunology
Life Sciences
Lymph Nodes
Receptors, CCR7
SciAdv r-articles
Synapses
Transcriptome
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Summary:Understanding the fate of dendritic cells (DCs) after productive immune synapses (postsynaptic DCs) with T cells during antigen presentation has been largely neglected in favor of deciphering the nuances of T cell activation and memory generation. Here, we describe that postsynaptic DCs switch their transcriptomic signature, correlating with epigenomic changes including DNA accessibility and histone methylation. We focus on the chemokine receptor as a proof-of-concept gene that is increased in postsynaptic DCs. Consistent with our epigenomic observations, postsynaptic DCs migrate more efficiently toward CCL19 in vitro and display enhanced homing to draining lymph nodes in vivo. This work describes a previously unknown DC population whose transcriptomics, epigenomics, and migratory capacity change in response to their cognate contact with T cells.
ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.abb9965